Amino acid sequence alignment of N. crassa CDT-1 and CDT-2 transporters using the PRALINE multiple sequence alignment program because of low sequence identity between CDT-1 and CDT-2 (Fig. S1), visualization of vacuoles (Fig. S2), localization of cells expressing CDT-1 or CDT-2 under normal conditions or after 6 hours of anaerobic growth (Fig. S3), I-TASSER model for CDT-2 (Fig. S4), Clustal Omega alignment of amino acid residues of FG and CDT-2, along with the truncated versions for both (Fig. S5), CDT-2-GFP expression levels (Fig. S6), and Clustal Omega protein sequence alignment (Fig. S7).
PDF, 4.5M