Supplemental Dataset 3: CLUSTAL W alignment of FKF1/ZTL proteins from Arabidopsis, Medicago, Soybean and Pea for Supplemental Figure 4. 1 10 20 30 40 50 60 | | | | | | | MtZTL (Medtr2g036510) -------------------------MEWDSNSDLSGDEDDAVSSS---FLLNDD----VG PsZTL (PsCam048993) -------------------------MEWDSNSDLSGDEDDAVSSSS--FLLNDD----VG GmZTL1a (Glyma.09G056100) -------------------------MEWDSNSDLS--DDEAVS-----FMLNDDDDDAVG GmZTL1b (Glyma.15G162300) -------------------------MEWDSNSDLS--DDDAVS-----YVLNDDDDDAVG GmZTL2a (Glyma.13G097600) -------------------------MEWDSNSDLSGDEEEEG------FVFNDGDDAGPL GmZTL2b (Glyma.17G062000) -------------------------MEWDSNSDLSGDEEEEG------FVFNDADDAGPL AtZTL (At5g57360) -------------------------MEWDSGSDLSADDASSLADD-----EEGGLFPGGG AtLKP2 (At2g18915) -------------------------MEWDSDSDLSGGDEVAEDG-----WFGGD----NG GmFKF1b (Glyma.05G239400) ---------------MAVTKEKEDVRNTGKRLKCMRNEEEE------------VYEEEES GmFKF1a (Glyma.08G046500) MGSSCLCSLFIFESDMAMPKEKEDVQNSGKRLKCTRNEEEEKEQYEQVEADEEEEEEEDS MtFKF1 (Medtr8g105590) ----MAMARDKKEDEEEVHNQNQKVGKRLKCMNMMKNEQEENQ----------VVDEEES PsFKF1 (PsCam049028) ----------------------------MLCMNMMKNEQENRV----------VDEEDES AtFKF1 (At1g18050) -----------------MAREHAIGEATGKRKKRGRVEEAEEYCN-----DGIEEQVEDE MtZTL (Medtr2g036510) PLPFPVLQ----TAPCGFVVTDALDPDHPIIYVNAVFEMLTGYRAEEVLGRNCRFLQCRG PsZTL (PsCam048993) PLPFPVLQTAQ-TAPCGFVVTDAIDPDHPIIYVNAVFEMLTGYRAEEVLGRNCRFLQCRG GmZTL1a (Glyma.09G056100) PLPFPVLQ----TAPCGFVVTDAVEPDHPIIYVNAVFEMVTGYRAEEVLGRNCRFLQCRG GmZTL1b (Glyma.15G162300) PLPFPVLQ----TAPCGFVVTDALDPDHPIIYVNAVFEMVTGYRAEEVLGRNCRFLQCRG GmZTL2a (Glyma.13G097600) PFPFPVVENLLQTAPCGFVVTDAHEPDNPIIYVNTVFEIVTGYRAEDVLGRNCRFLQCRG GmZTL2b (Glyma.17G062000) PFPFPVVENLLQTAPCGFVVTDALDPDHPIIYVNTVFEIVTGYCAEDVLGRNCRFLQCRG AtZTL (At5g57360) PIPYPVGN-LLHTAPCGFVVTDAVEPDQPIIYVNTVFEMVTGYRAEEVLGGNCRFLQCRG AtLKP2 (At2g18915) AIPFPVGS-LPGTAPCGFVVSDALEPDNPIIYVNTVFEIVTGYRAEEVIG---------- GmFKF1b (Glyma.05G239400) ELPLKPGFFFYPTTPTSFVVSDALEPDFPIIYVNKVFEISTGYRADEALGRNCRFLQYRD GmFKF1a (Glyma.08G046500) ELPLKLGLFFYPTIPTSFVVSDALEPDFPIIYVNKVFEIATGYRADEALGRNCRFLQYRD MtFKF1 (Medtr8g105590) ELPLKPELFFYPTTPTSFVVSDALESDFPIIYVNKVFEISTGYRAHEALGRNCRFLQYRD PsFKF1 (PsCam049028) ELPLKPEFFLYPTTPTSFVVSDALESDFPIIYVNKVFEISTGYRAHEALGRNCRFLQYRD AtFKF1 (At1g18050) KLPLEVGMFYYPMTPPSFIVSDALEPDFPLIYVNRVFEVFTGYRADEVLGRNCRFLQYRD MtZTL (Medtr2g036510) PFAKRRHPLVDSSVISEIRKCIDEGVEFQGELLNFRKDGSPLMNRLRLTPIYGEDDEITH PsZTL (PsCam048993) PFAKRRHPLVDSSVISEIRKCIDEGVEFQGELLNFRKDGSPLMNRLRLTPIYGEDDEITH GmZTL1a (Glyma.09G056100) PFAKRRHPLVDSTVVSEIRRCLDEGVEFQGELLNFRKDGSPLMNRLRLTPIYGED-EITH GmZTL1b (Glyma.15G162300) PFAKRRHPLVDSTVVSEIRRCLDEGVEFQGELLNFRKDGSPLMNRLRLTPIYGDG-EITH GmZTL2a (Glyma.13G097600) PFAKRRHPLVDSTVVSEIRRCLEEGIEFQGELLNFRKDGSPLMNRLRLTPIYGDDETITH GmZTL2b (Glyma.17G062000) PFAKRRHPLVDSTVVSEIRRCLEEGIEFQGELLNFRKDGSPLMNRLRLTPIYGDDETITH AtZTL (At5g57360) PFAKRRHPLVDSMVVSEIRKCIDEGIEFQGELLNFRKDGSPLMNRLRLTPIYGDDDTITH AtLKP2 (At2g18915) PFTKRRHPMVDSTIVAKMRQCLENGIEFQGELLNFRKDGSPLMNKLRLVPIREED-EITH GmFKF1b (Glyma.05G239400) PRAQRRHPLVDPVVVSEIRRCLEEGVEFQGELLNFRKDGTPLVNRLRLAPIHDDDGTVTH GmFKF1a (Glyma.08G046500) RRAQRRHPLVDPVVVSEIRRCLEEGVEFQGELLNFRKDGTPLVNRVRLTLIHDDDGTVTH MtFKF1 (Medtr8g105590) PRAQRRHPLVDPVVVSEIRRCLEEGIEFQGELLNFRKDGTPLVNRLRLTPIHDDDGVVTH PsFKF1 (PsCam049028) PRAQRRHPLVDPVVVSEIRRCLEDGIEFQGELLNFRKDGTPLVNRLRLTPIHDDDGIVTH AtFKF1 (At1g18050) PRAQRRHPLVDPVVVSEIRRCLEEGIEFQGELLNFRKDGTPLVNRLRLAPIRDDDGTITH MtZTL (Medtr2g036510) VIGIQLFTEANIDLGPLPGSTIKESLKSSGRFHSVLSSLQPPPLGDRNVS--RGICGIFQ PsZTL (PsCam048993) VIGIQLFTEANIDLGPVLGSTIKDSVKSSGRFRSVLSSLQPLPVGDRNVT--RGICGLFQ GmZTL1a (Glyma.09G056100) VIGIQFFTEANIDLGPLPGSTIKESTKSSDRFHSVLSSLNPVPVGDRNVT--RGICGIFQ GmZTL1b (Glyma.15G162300) VIGIQFFTEANIDLGPLPGSTIKESTKSSDRFHSVLSSLNPLPVGDRNVT--RGVCGILQ GmZTL2a (Glyma.13G097600) VIGIQFFTEANIDLGPVPGSTIKESAKSSDRFRSVLSSLQTLPVGGRNVS--RGVCGIFQ GmZTL2b (Glyma.17G062000) VIGIQFFTEANIDLGPVPGSTIKESAKSSDRFRSVLSSLQTLPVGDRNVS--RGVCGIFQ AtZTL (At5g57360) IIGIQFFIETDIDLGPVLGSSTKE--KSIDGIYSALA------AGERNVS--RGMCGLFQ AtLKP2 (At2g18915) FIGVLLFTDAKIDLGPSPDLSAKEIPRISRSFTSALP------IGERNVS--RGLCGIFE GmFKF1b (Glyma.05G239400) VIGIQLFSEANIDLNRVSYPVFKETCNQDFDKTGKYNPKSGQSLYSQHQ----EMCGILQ GmFKF1a (Glyma.08G046500) VIGIQLFSEANIDLNRVSYPVFKETCNQDFDKNGKYTPKSGQSLYSQHQ----EMCSILQ MtFKF1 (Medtr8g105590) IIGIQIFSEANIDLNRVSYPVFRETCIQDFDKNAKYSPKSGKLLYTPQK--REEMCGILQ PsFKF1 (PsCam049028) VIGIQIYSEANLDLNHISYPVYRETCIQDFDKNSKYSPKSGRLLYSPQQQQRREMCGILQ AtFKF1 (At1g18050) VIGIQVFSETTIDLDRVSYPVFKH--KQQLDQTSECLFPSGSPRFKEHH---EDFCGILQ MtZTL (Medtr2g036510) LSDEVLSLKILARLTPRDIASVSSVCTRLYEVTRNEDLWRMVCQNAWGSETTRVLETVPG PsZTL (PsCam048993) LSDEVLSLKILARLTPRDIASVSSVCTRLYEVTRNEDLWRMVCQNAWGSETTRVLETVPG GmZTL1a (Glyma.09G056100) LSDEVLSLKILARLTPRDIASVGSVCRHLYELTKNEDLWRMVCQNAWGSETTRVLETVPG GmZTL1b (Glyma.15G162300) LSDEVLSLKILARLTPRDIASVASVCRRLYELTENEDLWRMVCQNAWGSETTRVLETVPG GmZTL2a (Glyma.13G097600) LSDEVLSLKILAQLTPRDIASVSSVCRRLYELTKNEDLWRMVCQNAWGSETTHVLETVPG GmZTL2b (Glyma.17G062000) LSDEVLSLKILARLTPRDIASVSSVCRRLYELTKNEDLWRMVCQNAWGSETTRVLETVPG AtZTL (At5g57360) LSDEVVSMKILSRLTPRDVASVSSVCRRLYVLTKNEDLWRRVCQNAWGSETTRVLETVPG AtLPK2 (At2g18915) LSDEVIAIKILSQLTPGDIASVGCVCRRLNELTKNDDVWRMVCQNTWGTEATRVLESVPG GmFKF1b (Glyma.05G239400) LSDEVLAHNILSRLTPRDVASIGSVCRRIRQLTKNEHVRKMVCQNAWGKEVTGTLELMT- GmFKF1a (Glyma.08G046500) LSDEVLAHNILSRLTPRDVASIGSVCRRVRQLTKNEHVRKMVCQNAWGKEVTGTLELMT- MtFKF1 (Medtr8g105590) LSDEVLAHNILSRLTPRDVASIGSVCRRIRQLTKNEHVRKMVCQNAWGKEVTGTLELMT- PsFKF1 (PsCam049028) LSDEVLAHNILSRLTPRDVASIGSVCRRIRLLTKNEHVRKMVCQNAWGKEVTGTLELMT- AtFKF1 (At1g18050) LSDEVLAHNILSRLTPRDVASIGSACRRLRQLTKNESVRKMVCQNAWGKEITGTLEIMT- MtZTL (Medtr2g036510) AKRLGWGRLARELTTLEAAAWRKLTVGGGVEPSRCNFSACAVGNRVVLFGGEGVNMQPMN PsZTL (PsCam048993) ARRLGWGRLARELTTLEAAAWRKLTVGGGVEPSRCNFSACAVGNRVVLFGGEGVNMQPMN GmZTL1a (Glyma.09G056100) ARRLGWGRLARELTTLEAAAWRKLTVGGAVEPSRCNFSACAVGNRVVLFGGEGVNMQPMN GmZTL1b (Glyma.15G162300) ARRLGWGRLARELTTLEAAAWRKLTVGGAVEPSRCNFSACAVGNRVVLFGGEGVNMQPMN GmZTL2a (Glyma.13G097600) ARRLGWGRLARELTTLEAAAWRKLTVGGAVEPSRCNFSACAVGNRVVLFGGEGVNMQPMN GmZTL2b (Glyma.17G062000) ARGLGWGRLARELTTLEAAAWRKLTVGGAVEPSRCNFSACAVGNRVVLFGGEGVNMQPMN AtZTL (At5g57360) AKRLGWGRLARELTTLEAAAWRKLSVGGSVEPSRCNFSACAVGNRVVLFGGEGVNMQPMN AtLPK2 (At2g18915) AKRIGWVRLAREFTTHEATAWRKFSVGGTVEPSRCNFSACAVGNRIVIFGGEGVNMQPMN GmFKF1b (Glyma.05G239400) -KKLGWGRLTRELTTLEAVCWRKLTVGGAVEPSRCNFSACAAGNRLVLFGGEGVDMQPMD GmFKF1a (Glyma.08G046500) -KKMGWGRLTRELTTLEAVCWRKLTVGGAVEPSRCNFSACAAGNRLVLFGGEGVDMQPMD MtFKF1 (Medtr8g105590) -KKLGWGRLTRELTTLEAVCWKKVTVGGGVEPSRCNFSACAAGNRLVLFGGEGVDMQPMD PsFKF1 (PsCam049028) -KKLGWGRLSRELTTLEAVCWKKVTVGGGVEPSRCNFSTCAAGNRLVLFGGEGVDMQPMD AtFKF1 (At1g18050) -KKLRWGRLARELTTLEAVCWRKFTVGGIVQPSRCNFSACAVGNRLVLFGGEGVNMQPLD MtZTL (Medtr2g036510) DTFVLDLNSNNPEWQHVQVSSPPPGRWGHTLSCVNGSRLVVFGGCGTQGLLNDVFVLDLD PsZTL (PsCam048993) DTFVLDLNSNNPEWQHVQVSSPPPGRWGHTLSCVNGSRLVVFGGCGTQGLLNDVFVLDLD GmZTL1a (Glyma.09G056100) DTFVLDLNSSNPEWQHVQVSSPPPGRWGHTLSCVNGSRLVVFGGCGTQGLLNDVFVLDLD GmZTL1b (Glyma.15G162300) DTFVLDLNSSNPEWQHVQVSSPPPGRWGHTLSCVNGSRLVVFGGCGTQGLLNDVFVLDLD GmZTL2a (Glyma.13G097600) DTFVLDLNSSNPEWQHVQVSSPPPGRWGHTLSCVNGSHLVVFGGCGRQGLLNDVFVLDLD GmZTL2b (Glyma.17G062000) DTFVLDLNSSNPEWQHVHVSSPPPGRWGHTLSCVNGSHLVVFGGCGRQGLLNDVFVLDLD AtZTL (At5g57360) DTFVLDLNSDYPEWQHVKVSSPPPGRWGHTLTCVNGSNLVVFGGCGQQGLLNDVFVLNLD AtLKP2 (At2g18915) DTFVLDLGSSSPEWKSVLVSSPPPGRWGHTLSCVNGSRLVVFGGYGSHGLLNDVFLLDLD GmFKF1b (Glyma.05G239400) DTFVLNLDAKNPEWRRVSVKSSPPGRWGHTLSCLNGSWLVVFGGCGRQGLLNDVFVLDLD GmFKF1a (Glyma.08G046500) DTFVLNLDAKNPEWRRVSVKSSPPGRWGHTLSCLNGSWLVVFGGCGRQGLLNDVFVLDLD MtFKF1 (Medtr8g105590) DTFVLNLDAKNPEWQRVSVISSPPGRWGHTLSCLNSSWLVVFGGCGRQGLLNDVFVLDLD PsFKF1 (PsCam049028) DTFVLNLDAKNPEWERVIVKASPPGRWGHTISCLNSSWLVVFGGCGRQGLLNDVFVLDLD AtFKF1 (At1g18050) DTFVLNLDAECPEWQRVRVTSSPPGRWGHTLSCLNGSWLVVFGGCGRQGLLNDVFVLDLD MtZTL (Medtr2g036510) ATPPTWREISGLAPPLPRSWHSSCTLDGTKLIVSGGCADSGVLLSDTFLLDMSMENPVWR PsZTL (PsCam048993) ATPPTWREISGLAPPLPRSWHSSCTLDGTKLIVSGGCADSGVLLSDTFLLDMSMENPIWR GmZTL1a (Glyma.09G056100) AKPPTWREISGLAPPLPRSWHSSCTLDGTKLIVSGGCADSGVLLSDTFLLDLSMEKPVWR GmZTL1b (Glyma.15G162300) AKPPTWREISGLAPPLPRSWHSSCTLDGTKLIVSGGCADSGVLLSDTFLLDLSMEKPVWR GmZTL2a (Glyma.13G097600) AKPPTWREISGLAPPLPRSWHSSCTLDGTKLIVSGGCADSGVLLSDTFLLDLSMEKPVWR GmZTL2b (Glyma.17G062000) AKPPTWREISGLAPPLPRSWHSSCTLDGTKLIVSGGCADSGVLLSDTFLLDLSMEKPVWR AtZTL (At5g57360) AKPPTWREISGLAPPLPRSWHSSCTLDGTKLIVSGGCADSGVLLSDTFLLDLSIEKPVWR AtLPK2 (At2g18915) ADPPSWREVSGLAPPIPRSWHSSCTLDGTKLIVSGGCADSGALLSDTFLLDLSMDIPAWR GmFKF1b (Glyma.05G239400) AQQPTWREVCGGTPPLPRSWHSSCTIEGSKLVVSGGCTDAGVLLSDTYLLDLTTDNPTWR GmFKF1a (Glyma.08G046500) AQQPTWREVCGGTPPLPRSWHSSCTIEGSKLVVSGGCTDTGVLLSDTYLLDLTTDNPIWR MtFKF1 (Medtr8g105590) AQQPTWKEVFGEAPPLPRSWHSSCTIEGSKLVVSGGCTDAGVLLSDTYLLDLTIDNPTWR PsFKF1 (PsCam049028) AQQPTWKEVFGGAPPLPRSWHSSCTIEGSKLVVSGGCTDAGVLLSDTYLLDLTIDNPTWR AtFKF1 (At1g18050) AKHPTWKEVAGGTPPLPRSWHSSCTIEGSKLVVSGGCTDAGVLLSDTFLLDLTTDKPTWK MtZTL (Medtr2g036510) EIPVTWTPPSRLGHTLSVYGGRKILMFGGLAKSGPLRFRSSDVFTMDLSEDEPCWRCVTG PsZTL (PsCam048993) EIPVTWTPPSRLGHTLSVYGGRKILMFGGLAKSGPLRFRSSDVFTMDLSEEEPCWRCVTG GmZTL1a (Glyma.09G056100) EIPVAWTPPSRLGHTLSVYGGRKILMFGGLAKSGALRFRSSDVFTMDLSEEEPCWRCVTG GmZTL1b (Glyma.15G162300) EIPVAWTPPSRLGHTLSVYGGRKILMFGGLAKSGPLRFRSSDVFTMDLSEEEPCWRCVTG GmZTL2a (Glyma.13G097600) EIPVAWTPPSRLGHTLSVYGGRKILMFGGLAKSGPLRFRSSDVFTMDLSEEEPCWRCVTG GmZTL2b (Glyma.17G062000) EIPVAWTPPSRLGHTLSVYGGRKILMFGGLAKSGPLRFRSSDVFTMDLSEEEPCWRCVTG AtZTL (At5g57360) EIPAAWTPPSRLGHTLSVYGGRKILMFGGLAKSGPLKFRSSDVFTMDLSEEEPCWRCVTG AtLKP2 (At2g18915) EIPVPWTPPSRLGHTLTVYGDRKILMFGGLAKNGTLRFRSNDVYTMDLSEDEPSWRPVIG GmFKF1b (Glyma.05G239400) EIPTSWAPPSRLGHSLSVYGRTKILMFGGLAKSGHLRLRSGEAYTIDLEDEQPQWRQLEY GmFKF1a (Glyma.08G046500) EIPTSWAPPSRLGHSLSVYGRTKILMFGGLAKSGHLRLRSGEAYTIDLEDEQPQWRQLEY MtFKF1 (Medtr8g105590) EIPTSWTPPSRLGHSLSVYGRTKILMFGGLAKSGHLRLRSGEAYTIDLEAEQPQWRQLEC PsFKF1 (PsCam049028) EIPTPWTPPSRLGHSLSVYGRTKILMFGGLAKSGHLRLRSGDAYTIDLEAEQPQWRQLEC AtFKF1 (At1g18050) EIPTSWAPPSRLGHSLSVFGRTKILMFGGLANSGHLKLRSGEAYTIDLEDEEPRWRELEC MtZTL (Medtr2g036510) SGMP---GAGNPEGIAPPP-RLDHVAVSLPGGRILIFGGSVAGLHSASQLYILDPTDEKP PsZTL (PsCam048993) SGMP---GAGNPGGIAPPP-RLDHVAVSLPGGRILIFGGSVAGLHSASQLYILDPTDEKP GmZTL1a (Glyma.09G056100) SGLPGLPGTGNPGGIAPPP-RLDHVAVSLPGGRILIFGGSVAGLHSASQLYILDPTDEKP GmZTL1b (Glyma.15G162300) SGMP---GAGNPGGIAPPP-RLDHVAVSLPGGRILIFGGSVAGLHSASQLYILDPTDEKP GmZTL2a (Glyma.13G097600) SGMP---GAGNPGGIAPPP-RLDHVAVSLPGGRILIFGGSVAGLHSASQLYILDPTDEKP GmZTL2b (Glyma.17G062000) SGML---GAGNPGGTAPPP-RLDHVAVSLPGGRILIFGGSVAGLHSASQLYILDPTDEKP AtZTL (At5g57360) SGMP---GAGNPGGVAPPP-RLDHVAVNLPGGRILIFGGSVAGLHSASQLYLLDPTEDKP AtLKP2 (At2g18915) YGSS------LPGGMAAPPPRLDHVAISLPGGRILIFGGSVAGLDSASQLYLLDPNEEKP GmFKF1b (Glyma.05G239400) SAFTGL---ASQSAVVPPP-RLDHVAVSMPCGRIIIFGGSIAGLHSPSQLFLLDPSEEKP GmFKF1a (Glyma.08G046500) SAFTGL---ASQSAVVPPP-RLDHVAVSMPCGRIIIFGGSIAGLHSPSQLFLLDPSEEKP MtFKF1 (Medtr8g105590) SAFTGL---SNQNAVVPPP-RLDHVAVSMPCGRVIIFGGSIAGLHSPSQLFLLDPAEEKP PsFKF1 (PsCam049028) SAFTGL---ANQNAVVPPP-RLDHVAVSMPCGRVIIFGGSIAGLHSPSQLFLLDPSEEKP AtFKF1 (At1g18050) SAFPG--------VVVPPP-RLDHVAVSMPCGRVIIFGGSIAGLHSPSQLFLIDPAEEKP MtZTL (Medtr2g036510) TWRILNVPGRPPRFAWGHSTCVVGGTRAIVLGGQTGEEWMLSDLHELSLANSVI*--- PsZTL (PsCam048993) TWRILNVPGRPPRFAWGHSTCVVGGTRAIVLGGQTGDEWMLSELHELSLANSVI---- GmZTL1a (Glyma.09G056100) TWRILNVPGRPPRFAWGHSTCVVGGTRAIVMGGQTGEEWMLSELHELSLASSVS*--- GmZTL1b (Glyma.15G162300) TWRILNVPGCPPRFAWGHSTCVVGGTRAIVLGGQTGEEWMLSELHELSLASSVI*--- GmZTL2a (Glyma.13G097600) TWRILNVPGCPPRFAWGHSTCVVGGTRAIVLGGQTGEEWMLSELHELSLASSAI*--- GmZTL2b (Glyma.17G062000) TWRILNVPGCPPRFAWGHSTCVVGGTRAIVLGGQTGEEWMLSELHELSLASSAI*--- AtZTL (At5g57360) TWRILNIPGRPPRFAWGHGTCVVGGTRAIVLGGQTGEEWMLSELHELSLASYLT---- AtLKP2 (At2g18915) AWRILNVQGGPPRFAWGHTTCVVGGTRLVVLGGQTGEEWMLNEAHELLLATSTTAST- GmFKF1b (Glyma.05G239400) SWRILNVPGQPPKFAWGHSTCVVGGTRVLVLGGHTGEEWILNELHELCLASRQDSDL* GmFKF1a (Glyma.08G046500) SWRILNVPGQPPKFAWGHSTCVVGGTRVLVLGGHTGEEWILNELHELCLASRHDSDL* MtFKF1 (Medtr8g105590) TWRILNVPGEPPKFAWGHSTCVVGGTRVLVLGGHTGEEWVLNELHELCLASRQDSDM* PsFKF1 (PsCam049028) SWRILNVPGQPPKFAWGHSTCVVGGTRVLVLGGHTGEEWVLNELHELCLASRQDSDM- AtFKF1 (At1g18050) SWRILNVPGKPPKLAWGHSTCVVGGTRVLVLGGHTGEEWILNELHELCLASRQDSDL-