Folder = C:\Users\DELL\Desktop\ Name of the time-series = C:\Users\DELL\Desktop\movie2_fl.tif Start frame : End frame = 1:1:23 Distance unit = um Time unit = min Microscopy scaling factor = 0.264 um/pixel Time interval between two frames = 2 min LOI width = 1 pixel(s) Peak detection method = Findpeaks Peak search radius = 3 Minimum track length = 2 peaks Boolean to remove redundant tracks = 1 Splitting boolean = 1 omega1 = 5 Frames omega2 = 5 Pixels