Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie 1 - Supplemental movie. Examples of sperm motility patterns from the Ift20 mutants. Presented in the movie are short segments of freshly isolated, non-capacitated sperm from the control and the conditional Ift20 mutant mice. All segments were captured with a DAGE-MTI DC-330 3CCD camera and a Canon Optura 40 digital camcorder. Segments were assembled into the video using iMovie HD on a Dual 1GHz 414 PowerPC Processor G4 Apple Macintosh computer. A. A representative movie from a control mouse. Notice that most sperm were motile and displayed vigorous flagellar activity and progressive long track forward movement.
- Movie 2 - Supplemental movie. Examples of sperm motility patterns from the Ift20 mutants. Presented in the movie are short segments of freshly isolated, non-capacitated sperm from the control and the conditional Ift20 mutant mice. All segments were captured with a DAGE-MTI DC-330 3CCD camera and a Canon Optura 40 digital camcorder. Segments were assembled into the video using iMovie HD on a Dual 1GHz 414 PowerPC Processor G4 Apple Macintosh computer. B. A representative movie from a conditional Ift20 mutant mouse. Notice that there are less sperm compared to the control mouse in the same dilution, and only a small percentage of the mutant sperm were motile and showed forward motility, and flagellar movement was limited to a slow, erratic waveform with a very low amplitude.