Antibody-Based Immunotherapy To Treat and Prevent Infection with Hypervirulent Klebsiella pneumoniae

Supplemental material

• Supplemental file 1 -

  Supplemental methods.

  PDF, 80K

• Supplemental file 2 -

  Fig. S1. NET release by human neutrophils after stimulation with C. albicans, K1-Kp, or K1-Kp incubated with mAbs 4C5, 19A10, and IgG control. Fig. S2. Galleria mellonella survival and K1-Kp phagocytosis and killing by murine macrophages. Fig. S3. In vivo protection studies. Fig. S4. Intravital microscopy.

  PDF, 345K

• Supplemental file 3 -

  Video S1 to S3 legends.

  PDF, 37K

• Supplemental file 4 -

  Video S1. Three video clips for phagocytosis of K1-Kp pH-Rhodo-labeled cells by J744.16 are 2 h played at 5 fps, showing treatment with PBS, 4C5, or 19A10.

  MOV, 6.6M

• Supplemental file 5 -

  Video S2. Three video clips for i.v. injection are all 30 min postinfection played at 5 fps, showing treatment with 4C5, 19A10, or PBS.

  MOV, 2.7M

• Supplemental file 6 -

  Video S3. Three video clips for i.p. infection are 24 h postinfection played at 5 fps, showing treatment with 4C5, 19A10, or PBS.

  MOV, 2.3M