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Table 1. Levels of neuronal proteins in a –/– and a –/–/b –/– knockout mice
|
Protein |
a –/– |
a –/–, b –/– |
Antibody |
|
b -Synuclein |
100 ± 5 |
0.045 ± 0.12 |
U1127 |
|
b -Synuclein |
100 ± 1 |
–1.5 ± 0.8 |
Ab-1 |
|
a /b -Synuclein |
100 ± 8 |
–1.36 ± 1 |
Q698 |
|
a /b -Synuclein |
100 ± 16 |
–15.48 ± 4 |
U1126 |
|
b -Synuclein |
100 ± 3 |
0.4 ± 0.12 |
U1128 |
|
g -Synuclein |
100 ± 3 |
153 ± 5 |
SK23 |
|
14-3-3 z |
100 ± 5 |
72 ± 1 |
14-3-3 z |
|
14-3-3 e |
100 ± 5 |
136 ± 8 |
14-3-3 e |
|
Actin |
100 ± 3 |
71 ± 8 |
Actin (20-33) |
|
AP2 (a -adaptin) |
100 ± 9 |
113 ± 4 |
SC 7771 |
|
ApoE |
100 ± 6 |
120 ± 3 |
199-JH |
|
APP |
100 ± 1 |
91 ± 7 |
U955 |
|
Complexin II |
100 ± 1 |
130 ± 5 |
L668 |
|
Complexin I+II |
100 ± 5 |
134 ± 9 |
L669 |
|
CSP |
100 ± 9 |
91 ± 8 |
R807 |
|
DOC2 |
100 ± 4 |
97 ± 10 |
DOC2 |
|
Dynamin |
100 ± 5 |
97 ± 4 |
E027 |
|
Hsc 70 |
100 ± 4 |
111 ± 9 |
Hsc 70 |
|
Mint 1 |
100 ± 6 |
96 ± 7 |
U1692 |
|
Munc 18 |
100 ± 2 |
96 ± 4 |
K329 |
|
NMDAR |
100 ± 3 |
107 ± 3 |
54.1 |
|
NSF |
100 ± 5 |
96 ± 0.8 |
J372 |
|
Presenilin NTF |
100 ± 7 |
95 ± 3 |
NTF |
|
PSD-95 |
100 ± 1 |
109 ± 2 |
L667 |
|
Rab 3A |
100 ± 1 |
105 ± 8 |
42.2 |
|
Rim |
100 ± 4 |
104 ± 6 |
R809 |
|
SNAP 25 |
100 ± 3 |
96 ± 6 |
71.1 |
|
Synapsin Ia+b |
100 ± 9 |
105 ± 7 |
E028 |
|
Synaptotagmin I |
100 ± 3 |
92 ± 1 |
41.1 |
|
Synaptobrevin |
100 ± 11 |
99 ± 1 |
69.1 |
|
Synaptogyrin |
100± 3 |
123 ± 7 |
p 925 |
|
Synaptojanin |
100 ± 7 |
109 ± 7 |
T694 |
|
Synaptophysin |
100 ± 3 |
96 ± 12 |
43.1 |
|
Syntaxin |
100 ± 8 |
98 ± 9 |
HPC1 |
|
a -Tubulin |
100 ± 4 |
102 ± 4 |
a -Tubulin |
|
b -Tubulin |
100 ± 1 |
98 ± 8 |
N.357 |
|
t |
100 ± 5 |
88 ± 5 |
t |
|
Tyrosine hydroxylase |
100 ± 6 |
100 ± 5 |
s |
|
Veli |
100 ± 3 |
109 ± 5 |
T813 |
Levels of neuronal proteins in a –/– and a –/–/b –/– littermate mice (2 months old; n = 4) were determined by quantitative immunoblotting. Antibodies used are listed on the right. The changes in 14-3-3 proteins and complexins (shown in bold) were confirmed by analysis WT and a –/–/b –/– double-knockout (KO) mice (complexins, 50% increase; 14-3-3 z, 24% decrease in double KO compared with WT mice). All antibodies have been described (1) or are commercially available. The b -synuclein-specific antibody (Ab-1) was obtained from Oncogene Research Products (Cambridge, MA).