Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie 5 - MOVIE5_TEM: Transendothelial migration of lymphoblasts on a monolayer of inflamed HAEC visualized under 10x magnification. The transendothelial migration shown in Figure 7A is indicated using a yellow arrow. The movie is accelerated 150 times for a total true duration of 15 minutes. Scale bar is 50 μm.
- Movie 1 - MOVIE1_profile_microindentation: Profile microindentation of a resting T lymphocyte visualized under 100x magnification. The movie is accelerated 24 times for a total true duration of 16 minutes. Scale bar is 10 μm.
- Movie 2 - MOVIE2_MPA_resting_T_lymphocyte: Micropipette aspiration of a resting T lymphocyte visualized under 100x magnification. Brightfield and fluorescence images are combined. Bright signal indicates membrane rupture. The movie is accelerated 35 times. Scale bar is 10 μm.
- Movie 6 - MOVIE6_aspirated_Jurkat: Micropipette aspiration of a Jurkat cell visualized under 100x magnification. The aspiration pressure, initially of -100 Pa, is reversed halfway through the movie. The movie is accelerated 35 times. Scale bar is 10 μm.
- Movie 4 - MOVIE4_MPA_lymphoblast: Micropipette aspiration of a lymphoblast visualized under 100x magnification. Brightfield and fluorescence images are combined. Bright signal indicates membrane rupture. The movie is accelerated 35 times. Scale bar is 10 μm.
- Movie 3 - MOVIE3_MPA_Jurkat: Micropipette aspiration of a Jurkat cell visualized under 100x magnification. Brightfield and fluorescence images are combined. Bright signal indicates membrane rupture. The movie is accelerated 35 times. Scale bar is 10 μm.