Functional Roles of Acetylated Histone Marks at Mouse Meiotic Recombination Hot Spots

Supplemental material

• Supplemental file 1 -

  Summaries for Data Sets S1 to S3; Tables S1 (qRT-PCR primer pairs for probe sets of genes encoding HATs and HDACs), S2 (Primer pairs used to tile the HS59.5 hot spot), S3 (Sperm CO data at the HS59.5 hot spot), and S4 (Effects of HAT and HDAC inhibitors on CO rates); and Fig. S1 and S3 (Active and repressive histone H3 and H4 acetylated and methylated marks), S2 (H3K4Me3 mark ChIP profiles and nucleosome occupancy maps), S4, S5, S9, and S10 (Nonnormalized native ChIP profiles), S6 and S7 (Expression of genes encoding histone methylases and demethylases [S6] and acetylases and deacetylases [S7]), S8 and S11 (HDAC and HAT inhibitor effects on meiosis and global levels of acetylated histones [S8] and on histone acetylation marks [S11]), and S12 (Additional data supporting the proposed model of meiotic hot spot activation)

  PDF, 4.3M

• Supplemental file 2 -

  Data Set S1 (Differentially expressed genes encoding HAT, HDAC, HMT, and HDMT enzymes)

  XLSX, 112K

• Supplemental file 3 -

  Data Set S2 (Spo11^−/− versus wild‐type B6/DBA expression)

  XLSX, 85K

• Supplemental file 4 -

  Data Set S3 (9R motif sites specific for the PRDM9 9R allele)

  XLSX, 46K