The Type III Effector NleD from Enteropathogenic Escherichia coli Differentiates between Host Substrates p38 and JNK

Supplemental material

• Supplemental file 1 -

  Table S1. Complete list of pentapeptide mutants generated in pGFP-NleD using the mutation generation system kit F-701. Table S2. Single, double, and triple site-directed mutants made in pGFP-NleD. Fig. S1. Immunoblots of HT-29 cell lysates incubated with purified His-NleD, His-NleD_E143A, or His-NleD_R203E. Fig. S2. Peptide coverage map of GST-p38 and p38 cleavage products. Fig. S3. Extracted ion chromatograms showing the presence of the peptide ²¹³MAELLTGR²²⁰ within the C-terminal p38 cleavage product and the absence of the peptide within full-length GST-p38 and the N-terminal cleavage product.

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