Purification of UBC from a cell membrane and cytoplasmic fraction (Table S1); expression levels and conserved domains of Pelosinus sp. strain UFO1 genes with homology to the uranium-binding complex genes UFO1_4202 and UFO1_4203 (Table S2); purification of UBC from extracellular spent medium (Table S3); metal analysis of peak fraction from extracellular UBC size exclusion column (Table S4); most highly transcribed genes of Pelosinus sp. strain UFO1 (Table S5); Pelosinus sp. strain UFO1 genes with over 8-fold-increased expression in cells grown in the presence of 50 μM uranium (Table S6); Pelosinus strain UFO1 reduces anthraquinone-2,6-disulfonate (AQDS) to AH2DS during growth (Fig. S1); the uranium-binding complex (UBC) is present in the spent medium of cultures of Pelosinus sp. strain UFO1 (Fig. S2); reverse transcription-PCR of UBC genes (Fig. S3).
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