Complementation of flrA by a single copy results in recovery of bpfA expression in the ΔflrA mutant to the wild-type level (Fig. S1); FlrA is essential for the transcription of genes coding for flagella and consequent motility (Fig. S2); purified recombinant FlrA examined by SDS-PAGE (Fig. S3); determination of the transcriptional start site of the bpfA operon by fluorescence-based primer extension (Fig. S4); alignment of FlrA sequences from S. putrefaciens CN32, S. oneidensis MR-1, and V. cholerae with FleQ from P. aeruginosa PAO1 (Fig. S5); co-occurrence of FlrA and BpfA orthologs across the Shewanellaceae (Fig. S6).
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