Table 8. Oligonucleotide primers for amplifying 38 gene fragments and temperature used for denaturing HPLC

Locus

Primer

Sequence, 5-3¢

Primer

Sequence, 5-3¢

Temperature, ° C

YPO0142

O3446

GCTGAAGGCTCAGGGTAATG

O3447

CAGTACATCGGCACTGATCG

59.8

YPO0163

O3448

TAGGCACCTTCTTGGTCACC

O3449

ACTGACCAATTGGCCTGC

60.6

YPO0185

O3450

ACAGCTATTGGCCGGGTTAC

O3451

GGCGTAATGCTGTGTCACAC

60.0

YPO0256

O4584

GCATCCAGTGGTGTATTTT

O4585

CAGCAGCAGTGACAGTACTGA

64.5

YPO0282

O3452

ACAGGCTAAAGCAGAGCACC

O3453

GGAATAACCGGAGGTCCAAG

61.8

YPO0285

O3454

GAATAGATCGCCACTGGCTG

O3455

GTGCTCTGATATGGCCACTG

61.5

YPO0326

O3456

GTGCTCCTTGTAACCCACGT

O3457

GTTTGAGCCACCGCATTC

59.6

YPO0462

O4556

GGAACTGCAAGGCGATAATATTC

O4557

TAGTGCTGTTCAGCCCGTTTAAA

57.9

YPO0643

O3458

CCAGGAAGGCAACATTGG

O3459

CAGGCTCTCAGAAGTTGCAG

60.2

YPO0835

O4582

GCTACGTTAGTCGGAATCTG

O4583

GCATACCAACGCCCATTGC

62.0

YPO0858

O3460

ATGGCTTAGTAGGATCGGGG

O3461

CCCCTGGTTGGCTCATCTA

56.2

YPO0898

O4560

GCGCTGGCGGAAATCTTTA

O4561

GATGTCCAGTGCCAGCCACT

62.1

YPO1037

O3594

AAAATAAGGTGCGTCAAAG

O3595

CAGATCATCAAGGCACCGTAA

61.5

YPO1224

O3466

GTTGAAACGTCTCTCTGCCC

O3467

ATGCGGATCAGTGAGTCTGG

60.6

YPO1246

O3468

GAACACCTTCATCGAGCCTC

O3469

TCTGCGGAGCCAGTAATAGC

60.1

YPO1277

O3470

GCTGTGTTAATGGGCTACCG

O3471

GCCGTGATGATGTGTACCAG

61.1

YPO1291

O4562

CTGGAACGACCAACGCAATA

O4563

CGCAGCCGCAAGTAGTCTTT

62.9

YPO1372

O4564

CCGAGTGGGTCAGCTAATGG

O4565

ATTTACCACAACCAGTACGGCC

62.1

YPO1402

O3472

CCTTAGGCCATCAGGTGCT

O3473

GCTCAAGCCAACCGTATACC

60.7

YPO1526

O3474

CTGGAAGGGGCTGATATTCC

O3475

ACCGGTTAGGCGAGTTACCT

61.5

YPO1574

O4566

TGAAAAAGAAAACACGATCAAACATT

O4567

AGAGGTATCAATAGTAACAATCGCATAATT

56.0

YPO1581

O4568

AACGATTTAAGTTCACTGGGCG

O4569

TTTCAGTAGCGGGTAGCCGT

59.2

YPO2180

O3482

GAACTCCTTCAAGCCAGACG

O3483

CCTGGCCGTCAGAGTATTCA

59.4

YPO2291

O3484

CACTTTGGGACTCCCTTGC

O3485

CGAGTGGGATCTTCAGTTCC

59.5

YPO2420

O4570

AAGCGATTGTATTTGCCTATCATG

O4571

CTCAACCCACAAAGGGTGGT

60.6

YPO2439

O3486

CCGTGGTGGAGTCAATAACC

O3487

CTGTTCCGCAGGAATACGAG

62.3

YPO2612

O3488

CAGGTGCTGGCTGACTGTAC

O3489

CCTTTCTCAAGGGCTTCTGC

58.4

YPO2676

O4572

CGGTACCACGTCTGGCTGA

O4573

TTTTCATCATTTCTACTGCGCC

58.1

YPO2875

O4574

TGGCAGAGGAAGACCATCAAC

O4575

ACTGAATGCGGATTGGCG

60.0

YPO3120

O4576

AAGCCCGGCAGATATCCAT

O4577

GCTGTTGCATCCCCTTCAAC

60.2

YPO3268

O4578

CTAGCCAAGCGAGTCGGTG

O4579

CATACTTCGCTTCGCCGG

63.0

YPO3339

O3490

GATCACTACAGCTTGCCCGT

O3491

CTGCCCTTGCTCCGTATCT

56.4

YPO3352

O3492

AGGGTATCCCCGAGACCAT

O3493

GGAAGCCGTACAGATCGTGT

60.3

YPO3841

O3498

GGCAACTTGTTGGTACTGGC

O3499

CCGGATTACCGTAGCTCTCA

62.4

YPO3897

O3500

GTACCGTTCCCATACCACCA

O3501

AGAGTAGAGCATGCCACC

61.7

YPO3943

O3502

CCTTCAATGAAGCGGTCG

O3503

AACCCAGCTTACAGGTGGGT

62.1

YPO4007

O3504

GTCTGCGAGATAAGCCAAGC

O3505

AGAAGCAGCCTGCCTGTAAG

58.7

YPO4066

O4580

CACCCGGCATGAATATGAAGAT

O4581

TGGCGTTGCATAGCAATCAG

61.4

For YPO0256 and YPO1574 gradients were performed by using a time shift of 1.3 and 0.5 min, respectively.