BLASTP 2.3.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Stephen F. Altschul, John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. RID: 737JGRGA01R Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 77,704,984 sequences; 28,292,933,896 total letters Query= gi|81999730|sp|Q5UNX5.1|YL725_MIMIV RecName: Full=Uncharacterized protein L725 [Acanthamoeba polyphaga mimivirus] Length=224 Score E Sequences producing significant alignments: (Bits) Value ref|YP_003987254.1| hypothetical protein [Acanthamoeba polyph... 456 1e-160 gb|AKI80465.1| hypothetical protein [Acanthamoeba polyphaga m... 451 6e-159 ref|YP_007354190.1| hypothetical protein Moumou_00210 [Acanth... 255 1e-81 gb|AEX63069.1| hypothetical protein mv_R867 [Moumouvirus Monve] 254 3e-81 gb|AGF85542.1| hypothetical protein glt_00737 [Moumouvirus go... 248 1e-78 ref|WP_044997571.1| hypothetical protein [Ruminococcus sp. JC... 234 1e-73 ref|YP_004894330.1| mg279 gene product [Megavirus chiliensis] 234 2e-73 emb|CEO98163.1| hypothetical protein PBRA_006276 [Plasmodioph... 37.7 7.8 ALIGNMENTS >ref|YP_003987254.1| hypothetical protein [Acanthamoeba polyphaga mimivirus] sp|Q5UNX5.1|YL725_MIMIV RecName: Full=Uncharacterized protein L725 [Acanthamoeba polyphaga mimivirus] gb|AAV50985.1| unknown [Acanthamoeba polyphaga mimivirus] gb|ADO18369.1| hypothetical protein [Acanthamoeba polyphaga mimivirus] gb|AEJ34970.1| hypothetical protein MIMI_L725 [Acanthamoeba polyphaga mimivirus] gb|AEQ60937.1| hypothetical protein [Acanthamoeba castellanii mamavirus] gb|EJN41135.1| hypothetical protein lvs_L632 [Acanthamoeba polyphaga lentillevirus] gb|AHA45108.1| hypothetical protein HIRU_S202 [Hirudovirus strain Sangsue] gb|AHJ40340.1| hypothetical protein [Samba virus] gb|AKI79512.1| hypothetical protein [Acanthamoeba polyphaga mimivirus] gb|AKI81402.1| hypothetical protein [Acanthamoeba polyphaga mimivirus] Length=224 Score = 456 bits (1172), Expect = 1e-160, Method: Compositional matrix adjust. Identities = 224/224 (100%), Positives = 224/224 (100%), Gaps = 0/224 (0%) Query 1 MANNLVQLIFDQFIEILEDLAAKDEWCFDFNKCDFDFRVRELVNHRLLDVKYTIKDECGR 60 MANNLVQLIFDQFIEILEDLAAKDEWCFDFNKCDFDFRVRELVNHRLLDVKYTIKDECGR Sbjct 1 MANNLVQLIFDQFIEILEDLAAKDEWCFDFNKCDFDFRVRELVNHRLLDVKYTIKDECGR 60 Query 61 PRDVIQEIDITGICYEDLTTCKWVDYLTKLAVEYINNICPPRYIIIKEEPKKCRPQLPEW 120 PRDVIQEIDITGICYEDLTTCKWVDYLTKLAVEYINNICPPRYIIIKEEPKKCRPQLPEW Sbjct 61 PRDVIQEIDITGICYEDLTTCKWVDYLTKLAVEYINNICPPRYIIIKEEPKKCRPQLPEW 120 Query 121 NPFPCKRTTTIYRRQKPVEKKPECEVIFEKGCECLPSCEREVPVPKEQIFIKYEPVPAKC 180 NPFPCKRTTTIYRRQKPVEKKPECEVIFEKGCECLPSCEREVPVPKEQIFIKYEPVPAKC Sbjct 121 NPFPCKRTTTIYRRQKPVEKKPECEVIFEKGCECLPSCEREVPVPKEQIFIKYEPVPAKC 180 Query 181 CERTVLVRSPEQNRHSFGVHKGNIDYNNHVWPKCCQSKKCNCAH 224 CERTVLVRSPEQNRHSFGVHKGNIDYNNHVWPKCCQSKKCNCAH Sbjct 181 CERTVLVRSPEQNRHSFGVHKGNIDYNNHVWPKCCQSKKCNCAH 224 >gb|AKI80465.1| hypothetical protein [Acanthamoeba polyphaga mimivirus] Length=224 Score = 451 bits (1161), Expect = 6e-159, Method: Compositional matrix adjust. Identities = 221/224 (99%), Positives = 223/224 (99%), Gaps = 0/224 (0%) Query 1 MANNLVQLIFDQFIEILEDLAAKDEWCFDFNKCDFDFRVRELVNHRLLDVKYTIKDECGR 60 MANNLVQLIFDQFIEILEDLAAKDEWCFDFNKCDFDFRVRELVNHRLLDVKYTIKDECGR Sbjct 1 MANNLVQLIFDQFIEILEDLAAKDEWCFDFNKCDFDFRVRELVNHRLLDVKYTIKDECGR 60 Query 61 PRDVIQEIDITGICYEDLTTCKWVDYLTKLAVEYINNICPPRYIIIKEEPKKCRPQLPEW 120 PRDVIQEIDITGICYEDLTTCKWVDYL KLAVEYINNICPPRYIIIK+EPKKCRPQLPEW Sbjct 61 PRDVIQEIDITGICYEDLTTCKWVDYLNKLAVEYINNICPPRYIIIKDEPKKCRPQLPEW 120 Query 121 NPFPCKRTTTIYRRQKPVEKKPECEVIFEKGCECLPSCEREVPVPKEQIFIKYEPVPAKC 180 NPFPCKRTTTIYRRQKPVEKKPECEVIFEKGCECLPSCEREVPVPKEQIFIKYEPVPAKC Sbjct 121 NPFPCKRTTTIYRRQKPVEKKPECEVIFEKGCECLPSCEREVPVPKEQIFIKYEPVPAKC 180 Query 181 CERTVLVRSPEQNRHSFGVHKGNIDYNNHVWPKCCQSKKCNCAH 224 CERTVLVRSPEQNRHSFGVHKGNIDYNNH+WPKCCQSKKCNCAH Sbjct 181 CERTVLVRSPEQNRHSFGVHKGNIDYNNHIWPKCCQSKKCNCAH 224 >ref|YP_007354190.1| hypothetical protein Moumou_00210 [Acanthamoeba polyphaga moumouvirus] gb|AGC01754.1| hypothetical protein Moumou_00210 [Acanthamoeba polyphaga moumouvirus] Length=224 Score = 255 bits (651), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 124/223 (56%), Positives = 160/223 (72%), Gaps = 2/223 (1%) Query 1 MANNLVQLIFDQFIEILEDLAAKDEWCFDFNKCDFDFRVRELVNHRLLDVKYTIKDECGR 60 MANNL QLIFDQF+EI+E A + CFDF++CDF +RVRE VNHRL+DV Y +KD+CGR Sbjct 1 MANNLAQLIFDQFLEIVEKFAERS--CFDFDECDFRYRVREQVNHRLIDVLYEVKDKCGR 58 Query 61 PRDVIQEIDITGICYEDLTTCKWVDYLTKLAVEYINNICPPRYIIIKEEPKKCRPQLPEW 120 PRDV ID T IC EDL TCKWV+YL K+A E++ +ICP + I+K+EPKKCRPQLP+W Sbjct 59 PRDVYATIDFTSICLEDLVTCKWVEYLEKIAKEFVCDICPKKLAIVKQEPKKCRPQLPKW 118 Query 121 NPFPCKRTTTIYRRQKPVEKKPECEVIFEKGCECLPSCEREVPVPKEQIFIKYEPVPAKC 180 PFPCK TT+ R++ PV+ +PEC VI + CEC P CER VPK++I I+YEP + Sbjct 119 EPFPCKTVTTVIRKKVPVKVEPECHVIIKDDCECAPICERAPCVPKQEIIIQYEPESSCR 178 Query 181 CERTVLVRSPEQNRHSFGVHKGNIDYNNHVWPKCCQSKKCNCA 223 C +++ P+ +HSF +KG+ DYN+H W KK CA Sbjct 179 CGSNLVLVEPKHEKHSFKNYKGHPDYNHHEWNGNAGHKKAGCA 221 >gb|AEX63069.1| hypothetical protein mv_R867 [Moumouvirus Monve] Length=224 Score = 254 bits (649), Expect = 3e-81, Method: Compositional matrix adjust. Identities = 123/223 (55%), Positives = 160/223 (72%), Gaps = 2/223 (1%) Query 1 MANNLVQLIFDQFIEILEDLAAKDEWCFDFNKCDFDFRVRELVNHRLLDVKYTIKDECGR 60 MANNL QLIFDQF+EI+E A + CFDF++CDF +RVRE +NHRL+DV Y +KD+CGR Sbjct 1 MANNLAQLIFDQFLEIVEKFAERS--CFDFDECDFRYRVREQLNHRLIDVLYEVKDKCGR 58 Query 61 PRDVIQEIDITGICYEDLTTCKWVDYLTKLAVEYINNICPPRYIIIKEEPKKCRPQLPEW 120 PRDV ID T IC EDL TCKWV+YL K+A E++ +ICP + I+K+EPKKCRPQLP+W Sbjct 59 PRDVYATIDFTSICLEDLVTCKWVEYLEKIAKEFVCDICPKKLAIVKQEPKKCRPQLPKW 118 Query 121 NPFPCKRTTTIYRRQKPVEKKPECEVIFEKGCECLPSCEREVPVPKEQIFIKYEPVPAKC 180 PFPCK TT+ R++ PV+ +PEC VI + CEC P CER VPK++I I+YEP + Sbjct 119 EPFPCKTVTTVIRKKIPVKVEPECHVIVKDDCECAPVCERAPCVPKQEIIIQYEPESSCR 178 Query 181 CERTVLVRSPEQNRHSFGVHKGNIDYNNHVWPKCCQSKKCNCA 223 C +++ P+ +HSF +KG+ DYN+H W KK CA Sbjct 179 CGSNLVLVEPKHEKHSFKNYKGHPDYNHHEWNGNAGHKKAGCA 221 >gb|AGF85542.1| hypothetical protein glt_00737 [Moumouvirus goulette] Length=224 Score = 248 bits (632), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 121/223 (54%), Positives = 158/223 (71%), Gaps = 2/223 (1%) Query 1 MANNLVQLIFDQFIEILEDLAAKDEWCFDFNKCDFDFRVRELVNHRLLDVKYTIKDECGR 60 MANNL QLIFDQFIEI+E A K DF++CDF +RVRE +NHRL+DV Y +KD+CGR Sbjct 1 MANNLAQLIFDQFIEIVERFAEKSHC--DFDECDFRYRVREQLNHRLIDVLYEVKDKCGR 58 Query 61 PRDVIQEIDITGICYEDLTTCKWVDYLTKLAVEYINNICPPRYIIIKEEPKKCRPQLPEW 120 PRDV ID T IC EDL TCKWV+Y+ K+A E++ +ICP + I+K+EPKKCRPQLP+W Sbjct 59 PRDVYATIDFTSICLEDLVTCKWVEYIEKIAKEFVCDICPKKLAIVKQEPKKCRPQLPKW 118 Query 121 NPFPCKRTTTIYRRQKPVEKKPECEVIFEKGCECLPSCEREVPVPKEQIFIKYEPVPAKC 180 PFPCK TT+ R++ PV+ +PEC VI + CEC P CER VPK++I I+YEP P+ Sbjct 119 EPFPCKTVTTVIRKKIPVKVEPECHVIIKDDCECAPICERAPCVPKQEIIIQYEPEPSCR 178 Query 181 CERTVLVRSPEQNRHSFGVHKGNIDYNNHVWPKCCQSKKCNCA 223 C +++ P+ +HSF +KG+ D+N+H W K CA Sbjct 179 CGSNLVLVEPKHEKHSFKNYKGHPDFNHHEWNNKANHNKVGCA 221 >ref|WP_044997571.1| hypothetical protein, partial [Ruminococcus sp. JC304] Length=214 Score = 234 bits (597), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 122/216 (56%), Positives = 154/216 (71%), Gaps = 4/216 (2%) Query 1 MANNLVQLIFDQFIEILEDLAAKDEWCFDFNKCDFDFRVRELVNHRLLDVKYTIKDECGR 60 MANNL QLIF+QFIEILE A K ++C DF++CDF +RVRE VNHRL+DV Y +KD+CGR Sbjct 1 MANNLAQLIFNQFIEILERFAEK-KFCCDFDECDFRYRVREQVNHRLIDVLYEVKDKCGR 59 Query 61 PRDVIQEIDITGICYEDLTTCKWVDYLTKLAVEYINNICPPRYIIIKEEPKKCRPQLPEW 120 R+V ID T IC EDL TCKWV+YL +A E+I +ICP + I+K+EP+KCR QLP W Sbjct 60 VREVYATIDFTSICLEDLVTCKWVNYLETIAREFICDICPKKIAIVKQEPRKCRDQLPRW 119 Query 121 NPFPCKRTTTIYRRQKPVEKKPECEVIFEKGCECLPSCEREVPVPKEQIFIKYEP-VPAK 179 PFPCK TT+ R++K V +PEC VI + CEC P CER VPK++I I+YEP + Sbjct 120 EPFPCKTATTVIRKKKKVHVEPECHVIVKDECECAPICERVPCVPKQEIIIQYEPDTGCR 179 Query 180 CCERTVLVRSPEQNRHSFGVHKGNIDYNNHVWPKCC 215 C TVLV+ + +H F HKG D+N+H W KCC Sbjct 180 CGGNTVLVQD-KHEKHGFKDHKGKPDFNHHEW-KCC 213 >ref|YP_004894330.1| mg279 gene product [Megavirus chiliensis] ref|YP_007418162.1| hypothetical protein LBA_00266 [Megavirus lba] gb|AEQ32680.1| hypothetical protein [Megavirus chiliensis] gb|AEX61372.1| hypothetical protein c7_L306 [Megavirus courdo7] gb|AFX92316.1| hypothetical protein CE11_00286 [Megavirus courdo11] gb|AGD92186.1| hypothetical protein LBA_00266 [Megavirus lba] Length=225 Score = 234 bits (597), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 122/216 (56%), Positives = 154/216 (71%), Gaps = 4/216 (2%) Query 1 MANNLVQLIFDQFIEILEDLAAKDEWCFDFNKCDFDFRVRELVNHRLLDVKYTIKDECGR 60 MANNL QLIF+QFIEILE A K ++C DF++CDF +RVRE VNHRL+DV Y +KD+CGR Sbjct 1 MANNLAQLIFNQFIEILERFAEK-KFCCDFDECDFRYRVREQVNHRLIDVLYEVKDKCGR 59 Query 61 PRDVIQEIDITGICYEDLTTCKWVDYLTKLAVEYINNICPPRYIIIKEEPKKCRPQLPEW 120 R+V ID T IC EDL TCKWV+YL +A E+I +ICP + I+K+EP+KCR QLP W Sbjct 60 VREVYATIDFTSICLEDLVTCKWVNYLETIAREFICDICPKKIAIVKQEPRKCRDQLPRW 119 Query 121 NPFPCKRTTTIYRRQKPVEKKPECEVIFEKGCECLPSCEREVPVPKEQIFIKYEP-VPAK 179 PFPCK TT+ R++K V +PEC VI + CEC P CER VPK++I I+YEP + Sbjct 120 EPFPCKTVTTVIRKKKKVHVEPECHVIVKDECECAPICERVPCVPKQEIIIQYEPDTGCR 179 Query 180 CCERTVLVRSPEQNRHSFGVHKGNIDYNNHVWPKCC 215 C TVLV+ + +H F HKG D+N+H W KCC Sbjct 180 CGGNTVLVQD-KHEKHGFKDHKGKPDFNHHEW-KCC 213 >emb|CEO98163.1| hypothetical protein PBRA_006276 [Plasmodiophora brassicae] Length=460 Score = 37.7 bits (86), Expect = 7.8, Method: Compositional matrix adjust. Identities = 19/67 (28%), Positives = 31/67 (46%), Gaps = 1/67 (1%) Query 72 GICYEDLTTCKWVDYLTKLAVEYINNICPPRYII-IKEEPKKCRPQLPEWNPFPCKRTTT 130 G C + T WVD L + + +C P++II I P+LP + PF ++ Sbjct 216 GSCPLVVNTMGWVDGLGADLLAAVTGLCRPQHIIQISPSTDSAPPRLPSYRPFEFHHVSS 275 Query 131 IYRRQKP 137 R ++P Sbjct 276 QTRGKRP 282 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects Posted date: Dec 7, 2015 10:23 AM Number of letters in database: 28,292,933,896 Number of sequences in database: 77,704,984 Lambda K H 0.324 0.141 0.476 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 77704984 Number of Hits to DB: 14666527 Number of extensions: 13801 Number of successful extensions: 2186 Number of sequences better than 100: 6 Number of HSP's better than 100 without gapping: 0 Number of HSP's gapped: 2185 Number of HSP's successfully gapped: 6 Length of query: 224 Length of database: 28292933896 Length adjustment: 145 Effective length of query: 79 Effective length of database: 17025711216 Effective search space: 1345031186064 Effective search space used: 1345031186064 T: 21 A: 40 X1: 15 (7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (20.4 bits) S2: 76 (33.9 bits) ka-blk-alpha gapped: 1.9 ka-blk-alpha ungapped: 0.7916 ka-blk-alpha_v gapped: 42.6028 ka-blk-alpha_v ungapped: 4.96466 ka-blk-sigma gapped: 43.6362