Supplemental Materials and Methods: plasmid construction (molecular biology techniques, construction of M. florum oriC plasmids, construction of M. florum oriC plasmid derivatives, and construction of heterologous oriC plasmids); DNA sequence alignment of the intergenic regions upstream and downstream of dnaA in selected species of the Spiroplasma group (Fig. S1); growth curves of M. florum L1 wild-type strain and M. florum L1 carrying pMflTo4, pMflPT-o4, or pMflST-o4 in ATCC 1161 medium with or without the indicated antibiotics (Fig. S2); schematic representations of pMflT-o3 and pMflT-o4 plasmid recombination with the oriC region of the M. florum chromosome (Fig. S3); schematic representations of M. florum heterologous oriC plasmids (Fig. S4); primers used in this study (Table S1); compromise codon table for M. florum and E. coli (Table S2); E. coli and M. florum mating ratios for pMflT-o4 conjugation (Table S3); oriC region percentage identity matrix for selected species of the Spiroplasma group (Table S4); putative DnaA boxes found within the oriC intergenic regions of selected species of the Spiroplasma group (Table S5).
PDF, 3.5M