Mohler et al. 10.1073/pnas.0403711101.

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Fig. 6. Normal localization of cardiomyocyte proteins in 4 day old cultured adult rat cardiomyocytes. Image includes normal localization of markers for the intercalated disc (desmoplakin, ZO-1, β-catenin), sarcoplasmic reticulum (ryanodine receptor 2, SERCA2), and cardiomyocyte contractile apparatus (alpha-actinin).





Fig. 7. Voltage-gated Na (Nav) channel targeting in HEK293 cells does not require ankyrin-binding sequence. Hemagglutinin (HA)-tagged WT and mutant Nav1.5 and Nav1.2 were transfected (together with b subunit) into HEK293 cells. After 48 h, the cells were analyzed for Nav channel localization by using affinity-purified HA Ig. Mutants include Nav1.5 and Nav1.2 lacking the 9-aa ankyrin-binding sequence (ABS) and Nav1.5 E1053K Brugada mutant. There were no differences in plasma membrane fluorescence between the Nav channel constructs. Intracellular puncta were consistently observed in both WT and mutant cells. These data are consistent with an ankyrin-independent pathway for Nav channel targeting in noncomplex cells proposed by Garrido et al. (1).

1. Garrido, J. J., Giraud, P., Carlier, E., Fernandes, F., Moussif, A., Fache, M. P., Debanne, D. & Dargent, B. (2003) Science 300, 2091-2094.