Fig. S1. (A) In silico characterization of var gene primers used in the present study. (B) In silico comparison of new DBLα targeting primers to old versions published by Lavstsen et al. PNAS, 2012. Fig. S2. Overview of PfEMP1 domain classes, the estimated proportion of var genes encoding these, and predicted coverage of applied primer sets. Fig. S3. Transcript levels by primer sets reporting statistically significant different transcript levels between hospitalized patients with uncomplicated and severe malaria. Fig. S4. Median (25 and 75 percentiles, upper and lower adjacent values, and outliers) var transcript levels (Tu) reported in children with severe malarial anaemia (n = 44) or cerebral malaria (n = 29), with the primer set targeting DBL1/3-1. Table S1. Percentage of individuals of each patient group with var transcript levels higher than 16 Tu.
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