Scheme of Super-SAGE data analysis (Fig. S1); matching results of Super-SAGE tags with predicted genes and reference genome sequence of Lentinula edodes (Fig. S2); comparison between PCR-subtraction data and Super-SAGE data (Fig. S3); comparison of enriched gene ontology between fruiting body after harvest and fresh fruiting body by using Blast2GO enrichment analysis (Fig. S4); purification of GH16 enzyme, MLG1, using His-tag column, phenyl column, and MonoQ (Fig. S5); de novo assemble and scaffolding of the L. edodes genome (Table S1); number of CAZy enzymes involved in lignocellulose degradation in basidiomycetous fungi (Table S3); functional clustering of genes enriched in the fruiting bodies after harvest (Table S5) and those enriched in the fresh fruiting body (Table S6); differentially expressed oxidative stress, signal transduction, and cell death-related genes judged as DEG (Table S7).
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Comparison between recorded L. edodes genes and predicted L. edodes genes by BlastX (Table S2); comparison of Super-SAGE tag data between the fruiting body after harvest and the fresh fruiting body (Table S4).
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