Genome Editing in Clostridium saccharoperbutylacetonicum N1-4 with the CRISPR-Cas9 System

Supplemental material

• Supplemental file 1 -

  Primers (Table S1), gene deletion and mutant screening strategy in this study (Fig. S1), primers designed to detect the vector integration event during genome editing (Fig. S2), detection of pta deletion (Fig. S3 and S4), vector integration event detection (Fig. S5), and detection of buk deletion (Fig. S6 and S7).

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