New Quantitative Mass Spectrometry Approaches Reveal Different ADP-ribosylation Phases Dependent On the Levels of Oxidative Stress

Supplemental Data

• Suppl information (.pdf, 1.1 MB) - overview plus suppl figures
• Supplementary material: spectra (.pdf, 5.8 MB) - MS2 spectra of targeted ADP-ribosylated peptides. The fragments of ADP-ribose are indicated based on the nomenclature presented on the first page. The most intense fragment peaks are indicated
• Supplemental Table S1 (.xlsx, 74 KB) - The identified ADP-ribosylated proteins from shotgun measurements, including additional HeLa untreated shotgun identifications.
• Supplemental Table S2 (.xlsx, 106 KB) - The quantification of ADP-ribosylated proteins by LFQ measurements.
• Supplemental Table S3 (.xlsx, 191 KB) - The PRM method used to target ADP-ribosylated peptides in HeLa cells exposed to 4-64 ??M of H2O2 and obtained quantitative values.
• Supplemental Table S4 (.xlsx, 271 KB) - The PRM method used to target ADP-ribosylated peptides in SKOV3 and A2780 cells and obtained quantitative values.
• Supplemental Table S5 (.xlsx, 363 KB) - The PRM method used to target peptides in the cellular proteome of SKOV3 and A2780 cells to evaluate the abundance of the selected ADP-ribosylated proteins and obtained quantitative values.