Metformin directly binds the alarmin HMGB1 and inhibits its proinflammatory activity

Supplemental Data

• Supplemental Figure. 1-4 (.pdf, 446 KB) - Supplemental Figure 1. TLR4 inhibitor reduced HMGB1-induced p38 phosphorylation in RAW 264.7 cells Supplemental Figure 2. Metformin and anti-HMGB1 antibody induce change of gene expression in acetaminophen-induced liver injury model Supplemental Figure. 3 HMGB1 had no effect on the acetaminophen-induced injury of hepatocytes in vitro Supplemental Figure. 4: HMGB1 induced no inflammatory response in cultured hepatocytes