Evolutionary engineering process for the DS69473 strain (Fig. S1); growth of the DS69473 strains expressing the various Cyc8 Y353 mutants in 2% d-xylose and 12% d-glucose after 48 hours of aerobic cultivation (Fig. S2); normalized fold expression of the ADH1, FIT2, HXT10, HXT13, and HXT15-HXT16 genes (Fig. S3); glucose and xylose consumption and growth during mixed-sugar fermentation of DS68616-CYC8-Y353C and the wild type DS68616-CYC8-Y353 on 2% d-glucose and 2% d-xylose (Fig. S4); growth of the DS68625-derived strains on 2% d-glucose and 2% d-xylose + 0.05% d-maltose (Fig. S5); genomic sequencing data (Table S1); summary of transcriptomics data (Table S2); strains and plasmids used in this study (Table S3); oligonucleotides used in cloning and sequencing (Table S4) and in qPCR (Table S5).
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