Data analyzed: Microglia Source of Variation Degrees of Freedom Sum of Squares Mean square Treatment 6.0 23840000000 3974000000 Cytokine 41.0 621800000000 15170000000 Interaction 246.0 118900000000 483300000 Residual (error) 294.0 3345000000 11380000 Total 587.0 767900000000 Does Treatment have the same effect at all values of Cytokine? Interaction accounts for 15.48% of the total variance. F = 42.48. DFn=246 DFd=294 The P value is < 0.0001 If there is no interaction overall, there is a less than 0.01% chance of randomly observing so much interaction in an experiment of this size. The interaction is considered extremely significant. Since the interaction is statistically significant, the P values that follow for the row and column effects are difficult to interpret. Does Treatment effect the result? Treatment accounts for 3.11% of the total variance. F = 349.24. DFn=6 DFd=294 The P value is < 0.0001 If Treatment has no effect overall, there is a less than 0.01% chance of randomly observing an effect this big (or bigger) in an experiment of this size. The effect is considered extremely significant. Does Cytokine effect the result? Cytokine accounts for 80.98% of the total variance. F = 1332.86. DFn=41 DFd=294 The P value is < 0.0001 If Cytokine has no effect overall, there is a less than 0.01% chance of randomly observing an this big (or bigger) in an experiment of this size. The effect is considered extremely significant