Data analyzed: Astrocytes Source of Variation Degrees of Freedom Sum of Squares Mean square Treatment 6.0 40380000000 6730000000 Cytokine 41.0 910200000000 22200000000 Interaction 246.0 174400000000 709000000 Residual (error) 294.0 7422000000 25250000 Total 587.0 1132000000000 Does Treatment have the same effect at all values of Cytokine? Interaction accounts for 15.40% of the total variance. F = 28.08. DFn=246 DFd=294 The P value is < 0.0001 If there is no interaction overall, there is a less than 0.01% chance of randomly observing so much interaction in an experiment of this size. The interaction is considered extremely significant. Since the interaction is statistically significant, the P values that follow for the row and column effects are difficult to interpret. Does Treatment effect the result? Treatment accounts for 3.57% of the total variance. F = 266.59. DFn=6 DFd=294 The P value is < 0.0001 If Treatment has no effect overall, there is a less than 0.01% chance of randomly observing an effect this big (or bigger) in an experiment of this size. The effect is considered extremely significant. Does Cytokine effect the result? Cytokine accounts for 80.38% of the total variance. F = 879.38. DFn=41 DFd=294 The P value is < 0.0001 If Cytokine has no effect overall, there is a less than 0.01% chance of randomly observing an this big (or bigger) in an experiment of this size. The effect is considered extremely significant