Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Video 1 - Time sequence of phase contrast images of a melanophore microinjected with control non-immune IgG. Image acquisition started immediately after microinjection. After 20 min of recording, the cell was washed 5 times with tissue culture medium to remove excess MSH and melatonin added to induce aggregation of pigment granules. Microinjection of non-immune IgG did not significantly change the distribution of pigment granules, which aggregated in the cell center within about 20 min after melatonin treatment. Time stamp shows elapsed time in min:s. Bar, 5 μm.
- Video 2 - Time sequence of phase contrast images of a melanophore microinjected with TYRP1 antibodies. Image acquisition started immediately after microinjection. After 20 min of recording, the cell was washed 5 times with tissue culture medium to remove excess MSH and stimulated with melatonin to induce aggregation of pigment granules. Microinjection of TYRP1 antibodies induced the formation of clumps of pigment granules at the cell periphery. Upon stimulation with melatonin granule clumps moved to the cell center,but the rate of movement was slow and therefore the cell center remained devoid of pigment granules for an extended (30-40 min) period of time. Time stamp shows elapsed time in min:s. Bar, 5 μm.
- Video 3 - Time sequence of fluorescence images of the centrosome area of an EGFP-EB1-expressing melanophore injected with TYRP1 antibodies and stimulated with melatonin.An EGFP-EB1 comet (lower left corner) emerges from a clump of pigment granules and moves toward the centrosome (upper right corner), which is identified as a site of continuous birth of EGFP-EB1 comets. The direction of movement of the EGFP-EB1 comet indicates that the labeled MT was nucleated on an ectopic MT nucleation site that is likely associated with the pigment granule clump. Time stamp shows elapsed time in min:s. Bar, 2 μm.