A Wnt/Notch/Pax7 signaling network supports tissue integrity in tongue development

Supplemental Data

• Supplemental Figure S1 (.jpg, 1.5 MB) - Figure S1. X-gal staining showing Shh-Cre activity in E10.5 and E11.5 ShhCre/R26R embryonic tissues (A-D). Sections of stained samples show that in developing tongues Cre activity is limited in epithelium (E). L, limb; M, mandible; T, tongue; PS, palatal shelves. The arrow in C indicates positive staining in incisor.
• Supplemental Figure S2 (.jpg, 174 KB) - Figure S2. Quantification of the number of K5-positive (A) and P63-positive (B) basal cells in developing tongues. Data are shown as mean ????????? SD. **P < 0.01.
• Supplemental Figure S3 (.jpg, 1.5 MB) - Figure S3. TUNEL staining shows that cell apoptosis is not altered in Wls mutant tongues between E12.5 and E14.5. Scale bars: 200 ?????????m.
• Supplemental Figure S4 (.jpg, 873 KB) - Figure S4. In situ hybridization for the expression of MyoD1 and Myogenin in control and Wls mutant tongues at stages between E12.5 and E16.5. Scale bars, 500 ?????????m.
• Supplemental Figure S5 (.jpg, 171 KB) - Figure S5. Quantitative expression of Notch target genes in tongue explants cultured with or without DAPT. Data are shown as mean ????????? SD. **P < 0.01.
• Supplemental Figure S6 (.jpg, 148 KB) - Figure S6. Schematic diagram shows location of RBP-J binding sites (S1, S2) in the Pax7 regulatory region. Sequences of primers to detect the enrichment of S1 and S2 sites in the CHIP experiments are shown in the lower panels.