Table 1. Bacterial strains and plasmids used in this study

Strain or plasmid	Genotype or relevant characteristics	Ssource
E. coli strains
b 2150	F' lacZDM15 lacI^q traD36 proA⁺B⁺ thrB1004 pro thi strA hsdS lacZD M15 D dapA::erm (Erm^r) pir	7
b 2155	thrB1004 pro thi strA hsdsS lacZD M15 (F`lacZD M15 lacI^q traD36 proA⁺ proB⁺) D dapA::erm (Erm^r) pir::RP4 [::kan (Km^r) from SM10]	7
DH5α	F- (Φ80dlacZD M15) D (lacZY AargF)U169 deoR recA1 endA1 hsdR17 (rk^-, m_K⁺), sup E44 l -thi-1 gyrA96 relA1 l -CH616	4
NovaBlue	endA1 hsdR17(r K12–m K12+) supE44 thi-1 recA1 gyrA96 relA1 lac[F’ proA⁺B⁺ lacI^qZD M15::Tn10 (Tc^r)]	Novagen, Madison
A. tumefaciens strains
A. tumefaciens C58	wild-type, pTiC58, pAtC58	12
B. henselae strains
ATCC 49882T	‘Houston-1’, isolated from a bacteremic HIV-patient	13
MSE150	D bepA-G mutant of RSE247	This work
MS162	MS150 containing pRS51	This work
PGB69	RSE247 containing pPG104	This work
PGB70	RSE242 containing pPG104	This work
RSE242	D virB4 mutant of RSE247	1
RSE247	Spontaneous Sm^r strain of ATCC 49882T, serving as wild-type	1
RSE306	RSE247 containing pRS49	This work
RSE307	RSE247 containing pRS50	This work
RSE308	RSE247 containing pRS51	This work
RSE310	RSE247 containing pRS53	This work
RSE332	RSE242 containing pRS51	This work
RSE396	RSE247 containing pRS79	This work
RSE407	RSE247 containing pRS78	This work
RSE419	TRB148 containing pRS51	This work
RSE466	RSE247 containing pRS75	This work
RSE467	RSE247 containing pRS76	This work
RSE470	RSE247 containing pRS90	This work
RSE474	RSE247 containing pRS85	This work
RSE479	RSE247 containing pRS96	This work
RSE512	RSE247 containing pRS110	This work
RSE516	RSE247 containing pRS114	This work
RSE518	RSE247 containing pRS115	This work
RSE549	RSE247 containing pRS124	This work
TRB148	D virD4 mutant of RSE247	This work
TRB106	D bepB-G mutant of RSE247	This work
Plasmids
Cre-Stoplight	P_CMV, floxed sequence encoding DsRed, Cre-mediated recombination leads to expression of eGFP, AP^r	14
Cosmid BH14	pLAFR3 containing the bep region of B. henselae	This work
pATC58	Plasmid AT of A. tumefaciens strain C58	12
pBGR-K18	Fusion of pK18 and cryptic plasmid pBGR1 of B. grahamii, ori_ColE1, ori_pBGR1, Mob⁺,Tra^-, Km^r	15
pCB6	Expression plasmid carrying G418-resistance gene	Brewer and Roth, unpublished
pCD353	lacI^q, P_taclac, T_rrnB, gfp_mut2	8
pCHF3	E. coli- Bartonella spp. shuttle vector, ori_ColE1, ori_pBGR1, Tra^-, Km^r	15
pLAFR3	Tra^-, Mob⁺, Tc^r	6
pMS5	Derivative of pRS14 used to generate a D bepA-G in-frame deletion in B. henselae	This work
pPG100	Insertion of short sequence encoding a FLAG epitope into pRS40	This work
pPG104	Derivative of pPG100, encoding FLAG::BepD	This work
pRS14	oriT, ori_ColE1, gfp_mut2, lacI^q, rpsL, Km^r, mutagenesis vector for generating a D virB4 in-frame deletion in B. tribocorum	5
pRS15	Km^r of pCHF3 switched to Gm^r	This work
pRS29	Insertion of lacI^q and P_taclacinto pRS15	This work
pRS30	pRS29 expressing a NLS::Cre::MobA fusion protein	This work
pRS31	Insertion of T_rrnB into pRS30	This work
pRS39	pRS15 lacking one SalI side	This work
pRS40	pRS31 lacking one SalI side	This work
pRS49	Cre-vector encoding NLS::Cre::BepB (aa 303-542)	This work
pRS50	Cre-vector encoding NLS::Cre::BepC (aa 292-532)	This work
pRS51	Cre-vector encoding NLS::Cre::BepD (aa 352-534)	This work
pRS53	Cre-vector encoding NLS::Cre::BepF (aa 645-834)	This work
pRS56	Substitution of the DsRed coding sequence of CreStoplight	This work
	by a neomycin-resistance gene, Neo^r	This work
pRS75	Cre-vector encoding NLS::Cre::BepD (aa 485-534)	This work
pRS76	Cre-vector encoding NLS::Cre::BepD (aa 436-534)	This work
pRS78	Cre-vector encoding NLS::Cre::BepD (aa 352-485)	This work
pRS79	Cre-vector encoding NLS::Cre::BepD (aa 352-534) corresponding to pRS51 deleted for one AatII side	This work
pRS85	Cre-vector encoding NLS::Cre::BepF (aa 428-627)	This work
pRS90	Cre-vector encoding NLS::Cre::BepD (aa 352-514)	This work
pRS96	Cre-vector encoding NLS::Cre	This work
pRS108	Cre-vector encoding NLS::Cre::BepF (aa 428-627)	This work
pRS110	Cre-vector encoding NLS::Cre::BepF::BepD (aa 428-627; 497-534)	This work
pRS114	Cre-vector encoding NLS::Cre fused to aa 1348-1609 of TraA of plasmid pAT of A. tumefaciens C58	This work
pRS115	Cre-vector encoding NLS::Cre fused to aa 1485-1609 of TraA of plasmid pAT of A. tumefaciens C58	This work
pRS124	Cre-vector encoding NLS::Cre::BepD (aa 497-534)	This work
pSDM3204	Cre-vector encoding NLS::Cre::MobA	This work
pTR1000	pRS14 deleted for its BamHI-insert	This work
pTR1001	Pre-stage of pTR1003	This work
pTR1003	Derivative of pRS14 used to generate a D bepB-G in-frame deletion in B. henselae	This work
pTR1004	Derivative of pRS14 used to generate a D virD4 in-frame deletion in B. henselae	This work
pX1918GT	Cloning vector, Gm^r	16