Table 2. Oligonucleotide primers used in this study
|
Name |
Sequence |
Enzyme/Tag |
|
prAF2-113 |
GACTTCATCCAAGACAAACAA |
|
|
prAF2-120 |
TGCACCAGGCAATAAAAC |
|
|
prASB31 |
CGGAATTCGGTACCAAGACGTAGCCCAGGGG |
Eco RI |
|
prASB32 |
GGGGTACCAAGCTTGCATGCCTGCAG |
|
|
prASB51 |
CGGGATCCCCAATTACGAATTGACATAAGC |
Bam HI |
|
prPG89 |
CGCGAGCTCTTTAAGAAGGAGATATACAA |
Sac I |
|
ATGGACTACAAGGACGACGATGACAAGCATATGGGATGCCTGGCAGTTTAT |
FLAG, NdeI |
|
|
prPG91 |
CGCGAGCTCTAAATCAGAACGCAGAAGCG |
Sac I |
|
prPG98 |
GGAATTCCATATGAAAAAAAATCGACCATCCC |
NdeI |
|
prPG99 |
GGAATTCCATATGTTACATACCAAAGGCCATTCC |
NdeI |
|
prPG125 |
GCTCTAGACCTCGAAGATCTGGCAAATAA |
Xba I |
|
prPG126 |
CGTGTTTGCGATTTTTGCTGTTTGAGTGTTGTGGTGTTAGGAT |
prPG127-tag |
|
prPG127 |
ATCCTAACACCACAACACTCAAACAGCAAAAATCGCAAACACG |
prPG126-tag |
|
prPG128 |
GCTCTAGAGCGAATTGGGATCGAGAATA |
Xba I |
|
prRS91 |
CATGCCATGGATCTCGGCTTGAACGAATTG |
Nco I |
|
prRS106 |
GTTGGGAGTGAAATAAAACG |
|
|
prRS139 |
GTGACTTCAACGGTCATTTG |
|
|
prRS143 |
TATGCATCGAGCTCACAGGAGCGATATCATGG |
Sac I |
|
prRS144 |
TATGCATCGAGCTCCCCGGGCTACATGCTGAAATCTGGCC |
Sac I, XmaI |
|
prRS146 |
TTGTCGTTACCGGTTTAAATCAGAACGCAGAAGCG |
Age I |
|
prRS160 |
CCCCCCGGGATGCCTGGCAGTTTATGGC |
Xma I |
|
prRS169 |
TCCCCCCGGGTTAGCTGGCAATAGCAAGCG |
Xma I |
|
prRS171 |
TCCCCCCGGGTTAGTTGGTAAGAGCCCTTG |
Xma I |
|
prRS173 |
TCCCCCCGGGTTACATACCAAAGGCCATTCC |
Xma I |
|
prRS177 |
TCCCCCCGGGTTAGAGTGCCAGCACCATTTT |
Xma I |
|
prRS181 |
ATGGTGTCGACAAGAGCTTGAAAACACGCTC |
Sal I |
|
prRS182 |
ATGGTGTCGACTCGATCTGAAAGAAATATTGAT |
Sal I |
|
prRS183 |
ATGGTGTCGACAAAATCCCCTCTACGAAGG |
Sal I |
|
prRS185 |
ATGGTGTCGACAGCAAAAATCCCTTACACCTG |
Sal I |
|
prRS189 |
CGAGGAGCTCATTACCTCATATAGCATACATTATACGAAGTTAT |
Sac I, loxH |
|
AATTGCCCTTGCCACCATGATTGAACAAGATGGATTGC |
Kozak |
|
|
prRS190 |
CGAGGAGCTCTCAGAAGAACTCGTCAAGAAG |
Sac I |
|
prRS244 |
GCAACTCATCGATTGATTTAC |
Cla I |
|
prRS245 |
ATAGGACGTCGTTAGGCGCCC |
Aat II |
|
prRS247 |
GATAGTCGACGTGGACATGAGAGAGAGAC |
Sal I |
|
prRS248 |
GATAGTCGACAACTTGCGGGTCAAAAAATACTT |
Sal I |
|
prRS250 |
CCCCCCGGGTTAACGTTGTTTTTCCTGCTC |
Xma I, STOP |
|
prRS259 |
ATGGTGTCGACAACAAACACACAGAACGCAAAG |
Sal I |
|
prRS261 |
TCCCCCCGGGTTACAAAGTGCTCTCTTGTGTG |
Xma I, STOP |
|
prRS269 |
TATGCATCCCCGGGTTAATCGCCATCTTCCAGCAGG |
Xma I, STOP |
|
prRS271 |
ATGGTGTCGACAAGAGACGGTGCCGGTGTTG |
Sal I |
|
prRS272 |
ATGGTGTCGACGCCAGCGCATGAGCATTG |
Sal I |
|
prRS273 |
ATCCCACCGGTTCATTGGTTAAAGAGGATAACG |
Age I |
|
prRS276 |
TCCCCCCGGGCAAAGTGCTCTCTTGTGTG |
Xma I |
|
prRS278 |
TGATTACCGGTGAACACAGACATCATCGTCG |
Age I |
|
prRS280 |
GGACGAACGGAGTAAGTACT |
Sca I |
|
prRS349 |
TGATTGTCGACTTGAACACAGACATCATCGTCG |
Sal I |
|
prRS350 |
ATGGTACCGGTTTACATACCAAAGGCCATTCC |
Age I |
|
prRS351 |
ATGGTACCGGTAAAAATCCCCTCTACGAAGG |
Age I |
|
prRT1 |
ACGCGTCGACAAAGTAGTTTCTATGAGTAGATAA |
Sal I |
|
prTR2 |
CCGCTCGAGTGCAAATTTTTTAATCAGGATCAT |
Xho I |
|
prTR5 |
GCTCTAGATGGTATGTCCGTTCAGAACC |
|
|
prTR6 |
ACGCGTCGACAGAGGGATCCCTCAAATTCAT |
|
|
prTR33 |
GCTCTAGACCATGCAGTAAAACATGCAAG |
Xba I |
|
prTR34 |
CGTTCCAGTTACTTCAATGGTCATGGAAATGAGTGCCAGTTG |
pTR35-tag |
|
prTR35 |
ACCATTGAAGTAACTGGAACG |
|
|
prTR36 |
GCTCTAGACCATGAGAGTGTTAAATTCTTC |
Xba I |