Table 4. Immunoreactive proteins identified by serological screen of vaccinia proteome microarrays

Reactive in immunized mice, humans, and macaques

Antigen

PI

Mol. Wt.

Description

TM Domain/

Sig. Peptide

1

A10L

6.33

102,283

Major core protein

No/No

2

A11R

4.81

36,134

Hypothetical protein

Yes/No

3

A13L

9.96

7.696

Structural protein

Yes/Yes

4

A33R

5.3

20,506

EEV glycoprotein

Yes/Yes

5

A56R

4.05

34,778

Hemagglutinin

Yes/Yes

6

B5R

4.54

35,108

Plaque-size/host range protein

Yes/Yes

7

D8L

9.55

35,326

Cell surface-binding protein

Yes/No

8

D13L

5.10

61,890

Rifampicin resistance protein

No/No

9

F13L

6.98

41,823

Major envelope protein

No/No

10

H3L

6.43

37,458

IMV membrane protein

Yes/No

11

H5R

7.55

22,270

Late transcription factor

No/No

Reactive in Immunized Humans & Macaques

1

A26L

9.40

37,319

A-type inclusion protein

No/No

Reactive in Immunized Humans & Mice

1

A27L

5.14

12,616

Cell fusion protein

No/No

2

E3L

5.04

21,504

IFN resistance protein

No/No

3

L4R

6.13

28,460

DNA-binding core protein

No/No

Reactive in Immunized Mice & Macaques

1

H7R

7.27

16,912

Hypothetical protein

No/No

Reactive in Immunized Macaques Only

1

A17L

4.28

22,999

IMV membrane protein

Yes/Yes

Reactive in Immunized Mice Only

1

A3L

6.75

72,624

Major core protein

No/No

2

A4L

4.68

30,846

Memb. associated core protein

No/No

3

D11L

9.13

72,366

DNA helicase

No/No

4

H6R

10.30

36,665

topoisomerase

No/No

5

K2L

9.73

42,299

Serine protease inhibitor

No/Yes

6

N1L

4.41

13,961

Hypothetical proteins

No/No

Reactive in Naïve (Non-immunized) Humans

1

A41L

4.90

25,092

Secreted glycoprotein

No/Yes

2

A47L

10.29

28,334

Hypothetical protein

No/No

3

B2R

6.84

24,628

Hypothetical protein

No/No

4

D10R

8.12

28,934

NTP-phosphohydrolase

No/Yes

5

E1L

8.71

55,580

Poly(A) polymerase (VP55)

No/No

6

F2L

8.64

16,264

dUTP pyrophosphatase

No/No

7

F9L

6.72

23,792

Thioredoxin substrate

Yes/Yes

8

G5R

4.93

49,872

Core/assembly protein

No/No

9

G7L

7.72

41,920

Structural/core protein

No/No

10

H7R

7.27

16,912

Hypothetical protein

No/No

11

I1L

9,05

35,841

Telomere binding protein

No/No

12

L5R

10.32

15,044

Myristylated protein

Yes/No

13

O2L

5.27

12,355

glutaredoxin

No/No