Multalin version 5.4.1 Copyright I.N.R.A. France 1989, 1991, 1994, 1996 Published research using this software should cite Multiple sequence alignment with hierarchical clustering F. CORPET, 1988, Nucl. Acids Res., 16 (22), 10881-10890 Symbol comparison table: blosum62 Gap weight: 12 Gap length weight: 2 Consensus levels: high=90% low=50% Consensus symbols: ! is anyone of IV $ is anyone of LM % is anyone of FY # is anyone of NDQEBZ MSF: 1169 Check: 0 .. Name: Human Len: 1169 Check: 7166 Weight: 0.78 Name: Mouse Len: 1169 Check: 25 Weight: 0.60 Name: Rat Len: 1169 Check: 2539 Weight: 0.60 Name: Chicken Len: 1169 Check: 899 Weight: 1.25 Name: Carp Len: 1169 Check: 7748 Weight: 1.08 Name: Fugu Len: 1169 Check: 3239 Weight: 1.08 Name: Chimp Len: 1169 Check: 4610 Weight: 1.61 Name: Consensus Len: 1169 Check: 2111 Weight: 0.00 // 1 50 Human .......... ...MAPPSEE TPLIPQRSCS LLSTEAGALH VLLPARGPGP Mouse .......... ...MAPPSEE TPLIPQRSCS LSSSEAGALH VLLPPRGPGP Rat .......... ...MAPPSEE TPLISQRSCS LSSSEAGALH VLLPPRGPGP Chicken MACLTMANIE GSAIATVHQN GDILGNTSAP KQTEPMLQVY LYYSPGKTGG Carp ........MS EEEEVPLMKS ERAGSQKS.N WDS..ALQVH LYYSP.SLNS Fugu ......MNLA EEEFAPLVIR DRGGSQTS.S SSAGGSLQVH LYFVP.ATKK Chimp .......... ...MAPPSEE TPLIPQRSCS LLSTEAGALH VLLPARGPGP Consensus .......... ...mappsee tpli.#rScs l.s.eagalh vllpprgpgp 51 100 Human PQRLSFSFGD HLAEDLCVQA AKASGILPVY HSLFALATED LSCWFPPSHI Mouse PQRLSFSFGD YLAEDLCVRA AKACGILPVY HSLFALATED FSCWFPPSHI Rat PQRLSFSFGD YLAEDLCVRA AKACGILPVY HSLFALATED LSCWFPPSHI Chicken DY.LQFPAGE YVAEEICIVA CKACGIMPVY HNMFALMSET ERVWYPPNHI Carp ETTFTISTRH ITAENVCALA AKASGILPVF HNLFALASED LSYWYPPTHV Fugu ATTIHMTSGQ ISAENVCIQA GKKSGILPVY IGLFGLASAD LSFWYPPSHM Chimp PQRLSFSFGD HLAEDLCVQA AKASGILPVY HSLFALATED LSCWFPPSHI Consensus pqrlsfsfgd .lAE#lCv.A aKasGI$PV% hs$FaLated lscW%PPsHi 101 150 Human FSVEDASTQV LLYRIRFYFP NWF..GLEKC HRFGLRKDLA SAILDLPVLE Mouse FCIEDVDTQV LVYRLRFYFP DWF..GLETC HRFGLRKDLT SAILDLHVLE Rat FSIEDVDTQV LVYRLRFYFP GWF..GLETC HRFGLHKDLT SAILDVHVLE Chicken FHVDEATRLK LLYRIRFYFP HWYCNGTSRA YRYGIIRGSE SPVLDDLVMS Carp FKSEE..NIK VHYRVRFFFS SWFGQGSKAS YRFSLSRGRI CAVLDYCVID Fugu FNTEE..NLH VCFRVRFFFG NWFDQGPRTA CRYSLTRDRT SPVLDHSGIA Chimp FSVEDASTQV LLYRIRFYFP NWF..GLEKC HRFGLRKDLA SAILDLPVLE Consensus F.v##a.tqv ll%RiRF%Fp .W%..Gle.c hR%gl.kdl. sa!LD..vle 151 200 Human HLFAQHRSDL VSGRLPVGLS LKEQGECLSL AVLDLARMAR EQAQRPGELL Mouse HLFAQHRSDL VSGRLPVGLS MKEQGEFLSL AVLDLAQMAR EQAQRPGELL Rat HLFAQHRSDL VSGRLPVGLS LKDQGEFLSL AVLDLAQMAR KQAQRPGELL Chicken YLFAQWRKDF LDGWIQMPVT HETQEECLGM AVLDMMRVAK EKDQTPLAVY Carp YLFAQSRSDF VSGRGGISPA LSLQQECLGM AVLDLWRMAK ERNQSLGEIC Fugu YLFAQLRTDF TSSEAGLSPP LTTQDECLGL AVLDLWRLAK EQQSSVKDLC Chimp HLFAQHRSDL VSGRLPMGLS LKEQGECLSL AVLDLARMAR EQAQRPGELL Consensus hLFAQhRsDl vsgrlp.gls lk.QgEcLs$ AVLD$armAr Eqaqrpgell 201 250 Human KTVSYKACLP PSLRDLIQGL SFVTRRRIRR TVRRALRRVA ACQADRHSLM Mouse KTVSYKACLP PSLRDVIQGQ NFVTRRRIRR TVVLALRRVV ACQADRYALM Rat KSVSYKACLP PSLRDLIQGQ SFVTRRRIRR TVVQALAPCS SLPSRPYALM Chicken NSVSYKMFLP KCVRAKIQEY HILTRKRIRY RFNEFIQQFG QCKATARSAF Carp NTTSYKSCLP ETHRQDIQRI SRLARYQIRK TLKRFLKKLG RCSAVERILK Fugu KSVSYKSCLP KSHRVDIEKR NWVERFRIRN TLKHFLKKFG SCAVDDCNLK Chimp KTVSYKACLP PSLRDLIQGL SFVTRRRIRR TVRRALRRVA ACQADRHSLM Consensus ktvSYKacLP pslRd.I#g. sfvtRrrIRr tv..al.... .C.ad..slm 251 300 Human AKYIMDLERL DPAGAAETFH VGLPGAL..G GHDGLGLLRV AGDGGIAWTQ Mouse AKYILDLERL HPAATTETFR VGLPGA.... .QEEPGLLRV AGDNGISWSS Rat AKYILDLERL HPAATTESFL VGLPGA.... .QEEPGCLRV TGDNGIAWSS Chicken YSEVFEVKEP GGDPSGEESF ATIVIT.... ..GNGGIQCS RGKLKNCETL Carp LKYLMELSVL EPNYGSESFP LHHSGWLEQS EQQRVKAVRV SGEGGIQIQT Fugu LMYLTALAGI QPSLGSETFH VDPSSSCLNS .RSAVSLVQV TGEIGIQTSE Chimp AKYIMDLERL DPAGAAETFH VGLPGAL..G GHDGLGLLRV AGDGGIAWTQ Consensus akyi.dlerl .pa...Etf. vglpga.... .....gllrv .Gd.gi.wt. 301 350 Human G.EQEVLQPF CDFPEIVDIS IKQAPRVGPA GEHRLVTVTR TDNQILEAEF Mouse G.DQELFQTF CDFPEIVDVS IKQAPRVGPA GEHRLVTVTR MDGHILEAEF Rat K.DQELFQTF CDFPEIVDVS IKQAPRVGPA GEHRLVTITR MDGHILEAEF Chicken A.EQDL.QTY CDFPDIIDVS IKQASQEG.S SERRIVTIHK QDSKNLEAEF Carp K.ESQEWQTF CDFPQITDIS IKRLSQEQMP LEGRVVTLTR QDDQCMEAEF Fugu GCEGVVWQTF CDFKEITDIS IKRICREQVP NDSRMVTITR KDDACLEVAF Chimp G.EQEVLQPF CDFPEIVDIS IKQAPRVGPA GEHRLVTVTR TDNQILEAEF Consensus g.#qe..Qt% CDFp#IvD!S IKqaprvgpa g#hRlVT.tr .D..i$EaeF 351 400 Human PGLPEALSFV ALVDGYFRLT TDSQHFFCKE VAPPRLLEEV AEQCHGPITL Mouse PGLPEALSFV ALVDGYFRLI CDSRHYFCKE VAPPRLLEEE AELCHGPITL Rat PGLPEALSFV ALVDGYFRLI CDSRHFFCKE VAPPRLLEEE AELCHGPITL Chicken QSLREALSFV SLIDGYYRLT ADAHHYLCKE VAPPSVLENI QSNCHGPIFM Carp HTLTEALSFV SLVDGYFRLT TDSTHYSCAE VAPPSLLEDI QNYCHGPITS Fugu QSLKEALSLV SLVDGYFRLT TDSTHYFCQD TAPPSILEGI KNHCHGPITS Chimp PGLPEALSFV ALVDGYFRLT TDSQHFFCKE VAPPRLLEEV AEQCHGPITL Consensus pgLpEALSfV aL!DGY%RLt tDs.H%fCk# vAPPrlLEe. ae.CHGPItl 401 450 Human DFAINKLKTG GSRPGSYVLR RSPQDFDSFL LTVCVQ.NPL GPDYKGCLIR Mouse DFAIHKLKAA GSLPGTYILR RSPQDYDSFL LTACVQ.TPL GPDYKGCLIR Rat DFAIHKLKAA GSLPGSYILR RSPQDYDSFL LTACVQ.TPL GPDYKGCLIR Chicken DFAINKLKKA GNQTGFYVLR CSPKDFKKYF LTSAIE.REN ATDYKHCLIT Carp EFAVHKLKKA GAKNGMFLLR HSPKDFDKYF LTVCIQ.THL GMDYKDCLIE Fugu EFAVNKLKKW GSEDGTFLLR KSPQDYGEFF LTVSAAGTPL GLDYKDCLVI Chimp DFAINKLKTG GSHPGSYVLR RSPQDFDSFL LTVCVQ.TPL GPDYKGCLIR Consensus #FA!nKLK.a Gs.pG.%vLR rSPqD%ds%l LTvcvq.tpl gpDYKgCL!r 451 500 Human RSPTGTFLLV GLSRPHSSLR ELLATCWDGG LHVDGVAVTL TSCCIPRPKE Mouse QDPSGAFSLV GLSQPHRSLR ELLAACWNSG LRVDGAALNL TSCCAPRPKE Rat QDPSGAFSLV GLSQLHRSLQ ELLTACWHSG LQVDGTALNL TSCCVPRPKE Chicken KNENGEYNLS GTKRSFGNLK DLLTCYQTET VRSDSIIFQF IKCCPPKPKA Carp KNEK..FILA GIHNSFCSLK QLTDFYQHST LLMSDIPVTL GKCCPPRPKE Fugu KNKH..YSLP GVSKSFSSLK ELTVYYQQNK LLLADVPVRL SRCCPPRSQE Chimp RSPTGTFLLV GLSRPHSSLR ELLATCWDGG LHVDGVAVTL TSCCIPRPKE Consensus ..p.g.%.Lv Glsr.h.sL. #Ll..cw..g l.vdg.av.l tsCC.Prpke 501 550 Human KSNLI..... .......... ....VVQRGH SPPTSSLVQP QSQYQLSQMT Mouse KSNLI..... .......... ....VVRRGC TPAPAPGCSP .SCCALTQLS Rat KSNLI..... .......... ....VVRRGR NPTPAPGHSP .SCCALTKLS Chicken SEYVIFVFYG STFLLFLLPN KSNLLVFRSN SVSDVPSSPT LQRHNVSQMV Carp LTNMI..... .......... .....ILRNS SMTEMPSSPM LQRHKPSHMQ Fugu LTNLI..... .......... .....IMRNS SFVEAQLSPT LEGNKLIHIQ Chimp KSNLI..... .......... ....VVQRGH SPPTSSLVQP QSQYQLSQMT Consensus ksnlI..... .......... ....v!.Rg. sp...p...p .s...lsqm. 551 600 Human FHKIPADSLE WHENLGHGSF TKIYRGCRHE VVD.GEARKT EVLLKVMDAK Mouse FHTIPTDSLE WHENLGHGSF TKIFRGRRRE VVD.GETHDS EVLLKVMDSR Rat FHTIPADSLE WHENLGHGSF TKIFHGHRRE VVD.GETHDT EVLLKVMDSR Chicken FHKIRNEDLI FEESLGQGTF TKIFKGIRKE VGDYGQLHQT EVLLKVLDKV Carp FHMIKHEDLT WSESLGQGSF THIFRGSKTD QRD.GVTHTT EVLLKVLDAN Fugu FQMIKYEDLQ WGESHGQGSF TRLFKGYKSN LSE.GGKRVS .VFLKELDVN Chimp FHKIPADSLE WHENLGHGSF TKIYRGCRHE VVD.GEARKT EVLLKVMDAK Consensus FhkIp.#sLe whEnlGhGsF Tki%rG.r.# vv#.Ge.h.t eVlLKv$D.. 601 650 Human HKNCMESFLE AASLMSQVSY RHLVLLHGVC MAGD.STMVQ EFVHLGAIDM Mouse HRNCMESFLE AASLMSQVSY PHLVLLHGVC MAGD.SIMVQ EFVYLGAIDM Rat HQNCMESFLE AASLMSQVSY PHLVLLHGVC MAGD.SIMVQ EFVYLGAIDT Chicken HRNYSESFFE AASMMSQLSY KHLVLNYGVC VCGEENILVQ EYVKFGSLDT Carp HKNCWESFFE AASLMSQISH KHLLLVYGIS VHKSKNIMVQ EFVKHGALDL Fugu HRNLWESFFE AASLMSQFSH KHLILVYGVT L.AVKNIMVQ EFVEYGALDL Chimp HKNCMESFLE AASLMSQVSY RHLVLLHGVC MAGD.STMVQ EFVHLGAIDM Consensus H.NcmESFlE AAS$MSQvSy .HLvLlhG!c magd.si$VQ E%V.lGaiD. 651 700 Human YLRKRGHLVP ASWKLQVVKQ LAYALNYLED KGLPHGNVSA RKVLLAREG. Mouse YLRKRGHLVS ASWKLQVTKQ LAYALNYLED KGLPHGNVSA RKVLLAREG. Rat YLRKRGHLVP ASWKLQVTKQ LAYALNYLED KGLPHGNVSA RKVLLAREG. Chicken YLKKNKNVIN ILWKLEVAKQ LALAMHFLED KGLVHGNVCA KNILLIREED Carp YL.KKSTSVS VSWKLDVAKQ LACALNFLEE KNIAHGNICA KNLLLAREGD Fugu YL.KRGRSVS VSWKLDVAKQ LASVLTFLEQ NNIVHGNICA KNLLLAREGD Chimp YLRKRGHLVP ASWKLQVVKQ LAYALNYLED KGLPHGNVSA RKVLLAREG. Consensus YLrKrghl!. asWKL#V.KQ LAya$n%LE# kglpHGN!sA rkvLLaREg. 701 750 Human .ADGSPPFIK LSDPGVSPAV LSLEMLTDRI PWVAPECLRE AQTLSLEADK Mouse .GDGNPPFIK LSDPGVSPTV LSLEMLTDRI PWVAPECLQE AQTLGLEADK Rat .VDGNPPFIK LSDPGVSPTV LSLEMLTDRI PWVAPECLQE AGTLNLEADK Chicken RKSGNLPFIK LSDPGISITV LPRDILLERI PWVPPECIEN PRQLSLATDK Carp PSTDNSPFIK LSDPGINMAL LGKDVVLDRI PWVAPEVLDT LE.IELECDK Fugu PSQSIPPFIK LSDPGISVLM LGKDVIVDRI PWVAPEVLAS PENLTLESDK Chimp .ADGSPPFIK LSDPGVSPAV LSLEMLTDRI PWVAPECLRE AQTLSLEADK Consensus ..dgnpPFIK LSDPG!sp.v Lsl#mlt#RI PWVaPEcl.e a.tlsLeaDK 751 800 Human WGFGATVWEV FSGVTMPISA LDPAKKLQFY EDRQQLPAPK WTELALLIQQ Mouse WGFGATTWEV FSGGPAHITS LEPAKKLKFY EDQGQLPALK WTELAGLITQ Rat WGFGATTWEV FSGAPMHITS LEPAKKLKFY EDRGQLPALK WTELEGLIAQ Chicken WSFGTTLWEI CSGGDKPLSA LDSSRKLQFY EDRHQLPAPN WTELANLINN Carp WSFGTTLWEI FNNGEAPLQG QDLIHKQQFY EHFSNLPALE WTELADLIAQ Fugu WSFGATLWEL FNNGNNPLLG WDLDMK..FY ESFQQLPPSQ WTELADLINQ Chimp WGFGATVWEV FSGVTMPISA LDPAK..... .......... .......... Consensus WgFGaT.WEv fsgg..pisa l#pakk..fy e...qlpa.. wtela.li.q 801 850 Human CMAYEPVQRP SFRAVIRDLN SLISSDYELL SDPTPGALAP RDGLWNGAQL Mouse CMAYDPGRRP SFRAILRDLN GLITSDYELL SDPTPGIPSP RDELCGGAQL Rat CMAYDPGRRP SFRAILRDLN GLITSDYELL SDPTPGIPNP RDELCGGAQL Chicken CMDYEPDFRP SFRAIIRDLN SLFTPDYELL TE.NDMLPNI RTGALGFSGA Carp CMQYQPELRP SCRSIIRQLN SLITSDYEIL H..ATGTLPK SDGFWKRPNM Fugu CMDYQADFRP SCRSIIRQLF SLITSDYVIL F..ATE..PD TQFPAWRALT Chimp .......... .......... .......... .......... .......... Consensus cm.y.p..rp s.r.iir.ln slitsdye.l .......... .......... 851 900 Human YACQDPTIFE ERHLKYISQL GKGNFGSVEL CRYDPLGDNT GALVAVKQLQ Mouse YACQDPAIFE ERHLKYISLL GKGNFGSVEL CRYDPLGDNT GPLVAVKQLQ Rat YACQDPAIFE ERHLKYISLL GKGNFGSVEL CRYDPLGDNT GPLVAVKQLQ Chicken FEDRDPTQFE ERHLKFLQQL GKGNFGSVEM CRYDPLQDNT GEVVAVKKLQ Carp FKKQQQDVFE ERYLRFISIL GKGNFGSVEL CRYDPWGDNT GDLVAVKELQ Fugu PSQQEQTVFE ERHLRYISLL GKGNFGSVEL CCYDPLGDNT GELVAVKKLQ Chimp .......... .......... .......... .......... .......... Consensus ...q....fe erhl..is.l gkgnfgsvel crydplgdnt g.lvavk.lq 901 950 Human HSGPDQQRDF QREIQILKAL HSDFIVKYRG VSYGPGRQSL RLVMEYLPSG Mouse HSGPDQQRDF QREIQILKAL HSDFIVKYRG VSYGPGRQSL RLVMEYLPSG Rat HSGPEQQRDF QREIQILKAL HCDFIVKYRG VSYGPGRQEL RLVMEYLPSG Chicken HSTEEHLRDF EREIEILKSL QHDNIVKYKG VCYSAGRRNL RLIMEYLPYG Carp SNKQATVTDF QREIQTISSL HCDYIVKYKG ICYSTGRLST KLVMEYLPYG Fugu PSKKSSMEDF KKEVKTLSVL HCEYIVKYKG VCYSMGRLSM GLVTEYLPYG Chimp .......... .......... .......... .......... .......... Consensus .s......df .rei..l..l h.d.ivky.g v.y..gr.s. .lvmeylp.g 951 1000 Human CLRDFLQRHR ARLDASRLLL YSSQICKGME YLGSRRCVHR DLAARNILVE Mouse CLRDFLQRHR ARLHTDRLLL FAWQICKGME YLGARRCVHR DLAARNILVE Rat CLRDFLQRHR ARLHNDRLLL FAWQICKGME YLGARRCVHR DLAARNILVE Chicken SLRDYLQKHK ERLDHKKLLL YASQICKGME YLGTKRYVHR DLATRNILVE Carp SLIGYMEKHR QNVCTRRLLL FASQICKGME YLQSMRYVHR DLAARNILVA Fugu SLIGYLENNR HKVDTRRMLL FASQICKGME YLQSLRFVHR DLAARNILVA Chimp .......... .......... .......... .......... .......... Consensus .l...l..hr ......rlll .asqickgme yl...r.vhr dlaarnilv. 1001 1050 Human SEAHVKIADF GLAKLLPLDK DYYVVREPGQ SPIFWYAPES LSDNIFSRQS Mouse SEAHVKIADF GLAKLLPLGK DYYVVREPGQ SPIFWYAPES LSDNIFSRQS Rat SEAHVKIADF GLAKLLPLGK DYYVVRVPGQ SPIFWYAPES LSDNIFSRQS Chicken NENRVKIGDF GLTKVLPQDK EYYKVKEPGE SPIFWYAPES LTESKFSVAS Carp SDTLVKIADF GLTKIIPVDK EYYRVTQPGE SPVFWYAPES ISELKFSHKS Fugu SESLVKIADF GLTKIIPCNK EYYRVTQPGE SPIFWYAPES INESRFSHES Chimp .......... .......... .......... .......... .......... Consensus se..vkiadf gl.k..p..k .yy.v..pg. spifwyapes .....fs..s 1051 1100 Human DVWSFGVVLY ELFTYCDKSC SPSAEFLRMM GCERDVPALC .RLLELL.EE Mouse DVWSFGVVLY ELFTYCDKSC SPSAEFLSMM GPEREGPPLC .RLLELL.AE Rat DVWSFGVVLY ELFTYSDKSC SPSTEFLRMI GPEREGSPLC .HLLELL.AE Chicken DVWSFGVVLY ELFTYIEKSK SPPAEFMRMI GNDKQGQMIV FHLIELL.KN Carp DIWSFGIVLH ELFSYCDISR NPQKIVYPED RKLCPEVRPW LSIFLIFSKD Fugu DVWSFGVVLY ELFSYCDINS NPKRLYMQQI GHNVQTPSIS LHLANIL.KS Chimp .......... .......... .......... .......... .......... Consensus dvwsfgvvly elf.ycd.s. .p........ g......... ..l...l... 1101 1150 Human GQRLPAPPAC PAEVHELMKL CWAPSPQDRP SFSALGPQLD .MLWSGSRGC Mouse GRRLPPPPTC PTEVQELMQL CWAPSPHDRP AFGTLSPQLD .ALWRGRPG. Rat GRRLPPPSTC PTEVQELMQL CWSPNPQDRP AFDTLSPQLD .ALWRGSPG. Chicken NGRLPRPDGC PDEIYAIMKE CWNNNVAQRP TFRDLAQRVD .QIRDNMGG. Carp NWRLPAPSQC PPKVHNIMMQ CWEFNSEDRP SFSSLQDLIE NSLLDEREGC Fugu NWRLPAPPDC PAKVYSLMKE CWLYDFMERP CFSFLGNQIE VIMQDERDSF Chimp .......... .......... .......... .......... .......... Consensus ..rlp.p..c p..v...m.. cw......rp .f..l..... ........g. 1151 1169 Human ETHAFTAHPE GKHHSLSFS Mouse .......... ......... Rat .......... ......... Chicken .......... ......... Carp KGKIRVTLNR SLYFQHMYI Fugu KG........ ......... Chimp .......... ......... Consensus .......... .........