Table 2. Relevant features and homologues of proteins of the Staphylococcus aureus Ess cluster

Gene name (Mu50)	Protein	Length, aa	Predicted location*		Subcellular location^†	Homologues
Gene name (Mu50)	Protein	Length, aa	Soluble	TMD TS	Subcellular location^†	Gene	Length, aa	E value
SAV0282	EsxA	97	Yes	0	Medium	Lmo0056 SAG1039 CAC3713 BS_yukE	97 96 96 80	3.5e-23 1.4e-19 6e-9 0.32
SAV0283	EsaA	1,009	No		ND	Lmo0057 SAG1038 CAC3696	1,068 1,003 941	5.8e-67 3.2e-65 5e-21
SAV0284	EssA	152	No	1	ND	Lmo0058	171	0.036
SAV0285	EsaB	80	Yes	0	ND	Lmo0059 SAG1036 BS_yukD	83 80 79	1.5e-11 0.013 0.048
SAV0286	EssB	444	No	1	ND	Lmo0060 SAG1035 BS_yukC	398 424 451	4.1e-50 4e-37 7.8e-25
SAV0287-0288^‡	EssC	1,479	No	2	ND	Lmo0061 SAG1033 BS_yukAB	1,498 1,309 1,498	0 0 0
SAV0289	EsxY	130	Yes	0	ND	Lmo0062?	131	none
SAV0290	EsxB	104	Yes	0	Medium	Lmo0063 BC3306	99 101	1.9e-5 0.011

TMD, transmembrane domain; TS, topogenic signal; ND, not determined; Lmo, Listeria monocytogenes; BS, Bacillus subtilis; CAC, Clostridium acetobutylicum; SAG, Streptococcus agalactiae; BC, Bacillus cereus. Subcellular location of the proteins was predicted using the TMM algorithm (*) or established experimentally (†), as described in the text.

^{‡Because of a sequencing error, EssC appears to be encoded by two genes (SAV0287-0288) in the Mu50 genome.}