Table 2. Splenic and hepatic leukocyte numbers from WT mice treated with clodronate (CL2) or PBS liposomes

Organ	Cell type	Treatment	Percentages	Absolute no. (´ 10⁶)
Spleen	DCs	PBS	1.4 ą 0.3	1.5 ą 0.3
Spleen	DCs	CL2	1.0 ą 0.2	1.1 ą 0.2
Spleen	Macrophages	PBS	5.2 ą 0.4	5.7 ą 0.4
Spleen	Macrophages	CL2	4.1 ą 0.2	4.5 ą 0.2
Spleen	NK cells	PBS	2.0 ą 0.5	2.2 ą 0.6
Spleen	NK cells	CL2	1.8 ą 0.4	2.0 ą 0.4
Spleen	NKT cells	PBS	1.7 ą 0.2	1.9 ą 0.2
Spleen	NKT cells	CL2	1.7 ą 0.3	1.9 ą 0.3
Spleen	Other T cells	PBS	35 ą 3	39 ą 3
Spleen	Other T cells	CL2	33 ą 3	36 ą 3
Spleen	B cells	PBS	60 ą 3	66 ą 3
Spleen	B cells	CL2	65 ą 4	72 ą 4
Spleen	Marginal zone B cells	PBS	5 ą 0.8	6 ą 0.9
Spleen	Marginal zone B cells	CL2	6 ą 0.5	7 ą 0.6
Liver	DCs	PBS	0.45 ą 0.05	0.16 ą 0.002
Liver	DCs	CL2	0.39 ą 0.08	0.14 ą 0.003
Liver	Kupffer cells	PBS	5.2 ą 0.6	0.18 ą 0.02
Liver	Kupffer cells	CL2	0.5 ą 0.1	0.018 ą 0.004
Liver	NK cells	PBS	14 ą 3	0.5 ą 0.1
Liver	NK cells	CL2	13 ą 2	0.5 ą 0.07
Liver	NKT cells	PBS	12 ą 4	0.5 ą 0.15
Liver	NKT cells	CL2	13 ą 3	0.5 ą 0.1
Liver	Other T cells	PBS	35 ą 6	1.2 ą 0.2
Liver	Other T cells	CL2	38 ą 4	1.3 ą 0.15
Liver	B cells	PBS	50 ą 4	1.8 ą 0.15
Liver	B cells	CL2	48 ą 3	1.7 ą 0.2

Forty-eight hours after liposome administration, the percentages of each cell type were obtained by means of FACS surface staining and analysis, as explained in Materials and Methods. The absolute numbers of each cell type were calculated based on the FACS percentages and the total number of leukocytes obtained from the spleens and livers of each mouse. Five WT mice were used for each treatment, and the numbers shown represent the average ą SD of each cell type from the five mice. The data shown are from one representative experiment of three.