The Role of Microtubules in Rapid Hyphal Tip Growth of Aspergillus nidulans Mol. Biol. Cell Horio and Oakley 16: 918
Supplemental Material
This article contains the following supporting material:
Fig1.mov - (corresponds to Figure 1). Growth and mitosis in a binucleate germling. Microtubules are labeled with GFP-α-tubulin. Images are projections of Z-series stacks captured with a wide field microscope at three min intervals. Playback is five frames per second. As the germling enters mitosis two mitotic spindles assemble as cytoplasmic microtubules disassemble. Cytoplasmic microtubules reassemble after mitosis. Tip growth is slow but accelerates after mitosis.
Fig2.mov (1.19 MB) - (corresponds to Figure 2). The effect of benomyl on growth of two hyphal tip cells. Microtubules are labeled with GFP-α -tubulin. Images are projections of Z-stacks obtained with a wide field microscope. Images were captured at three minute intervals and playback is at five frames per second. The germling grows and after benomyl is added the microtubules disassemble and growth slows. The hyphal tips bulge and a secondary bulge forms in the hypha at the left.
Fig3.mov - (corresponds to Figure 3). Recovery of tip growth after benomyl wash out. Images are projections of Z-stacks obtained with a wide field microscope. Images were captured at three minute intervals and playback is at five frames per second. Microtubules are already visible in the first image and a normal microtubule array quickly forms. The bulge at the hyphal tip enlarges and then a normal hyphal tip extends.
Fig4.mov - (corresponds to Figure 4). Breakdown of cytoplasmic microtubules in the central portion of a tip cell. Images are projections of Z-stacks obtained with a spinning disk confocal microscope. Images were captured at 33.5 seconds intervals. Playback is at five frames per second. A few remnant cytoplasmic microtubules are present in the first image but they quickly disassemble. Three spindles complete mitosis and astral microtubules assemble from the spindle pole bodies.
Fig5a.mov (1.27 MB) - (corresponds to Figure 5A). Cytoplasmic microtubules do not break down completely in mitosis near the hyphal tip. Images are projections of Z-stacks obtained with a spinning disk confocal microscope. Images were captured at 30 seconds intervals. Playback is at five frames per second. Cytoplasmic microtubules do not disassemble completely before astral microtubules assemble.
Fig5c.mov - (corresponds to Figure 5C). Cytoplasmic microtubules remain intact near the hyphal tip in mitosis. Images are Z-stacks obtained with a spinning disk confocal microscope. Images were captured at 30 seconds intervals. Playback is at five frames per second cytoplasmic microtubules do not disassemble completely before astral microtubules assemble.