|
Parhar et al. 10.1073/pnas.0409494102. |
Table 2. Sequences of PCR primers and fluorogenic probes used for PCR
|
Primer |
Sequence |
Code |
|
RT-PCR |
|
|
|
GH1-F |
5'-ATTATCAAAGTCTGGGAGGC-3' |
GH1 |
|
GH2-F |
5'-ATTATCAAAGTCTGGGAGGG-3' |
GH2 |
|
GH-R |
5'-GGTAGGTCTCCACCTTGTGC-3' |
GH3 |
|
FSHβ-F |
5'-CCAGAAGGATCCCAACTTCA-3' |
FSHβ 1 |
|
FSHβ-R |
5'-CAGAGGGAAGAGGTGCAAAG-3' |
FSHβ2 |
|
LHβ-F |
5'-GACACTGCATCACCAAGGAC-3' |
LHβ1 |
|
LHβ-R |
5'-GGTTGCATGCTCTCAAAGGT-3' |
LHβ2 |
|
TSHβ-F |
5'-TCAACACCACCATCTGCATGGG-3' |
TSHβ1 |
|
TSHβ-R |
5'-AGGGCCACGGGGTAGGTGAAG-3' |
TSHβ2 |
|
PRL188-F |
5'-GCAACAGCTAACGGACAGAA-3' |
PRL1 |
|
PRL188-R |
5'-CTGAGCAGGTGAACCCATCT-3' |
PRL2 |
|
PRL177-F |
5'-AGCTGGGCTCTGAAGCATT-3' |
PRL3 |
|
PRL177-R |
5'-AGCTGGACCCATCTTGCTA-3' |
PRL4 |
|
POMC-F |
5'-GCAACATGATGGAGTGCATC-3' |
POMC1 |
|
POMC-R |
5'-CAGCGGAAGTGCTTCATCTT-3' |
POMC2 |
|
SL-F |
5'-TCAGCTGCTTCAAGAAAGA-3' |
SL1 |
|
SL-R |
5'-GAAATCAATGGCAGGATATG-3' |
SL2 |
|
GnRHR1-F |
5'-CGACTGAAAAAGGACAATTT-3' |
GR1 |
|
GnRHR1-R |
5'-GCGCAACTAAAAGACCCAGT-3' |
GR2 |
|
GnRHR1-R2 |
5'-GAGTTCTCATCCGGGCTCTC-3' |
GR3 |
|
GnRHR2-F |
5'-CAGCACCTGAGAGACAAAGC-3' |
GR4 |
|
GnRHR2-F2 |
5'-CCATACTACCTGCTGGGGATT-3' |
GR5 |
|
GnRHR2-R |
5'-ATGTATTGGGGCGAAGTGTC-3' |
GR6 |
|
GnRHR3-F |
5'-TGCTACACCAGGATCTTTATTCAGA-3' |
GR7 |
|
GnRHR3-R |
5'-GGCAGGCATTAAACAGACCAA-3' |
GR8 |
|
GnRHR3-R2 |
5'-CTTTCCTTCCAGGTCATCT-3' |
GR9 |
|
RT-Q-RT-PCR |
|
|
|
Q-GnRHR1-F |
5'-TCCAGACGACTGAAAAAGGACA-3' |
GR10 |
|
Q-GnRHR1-R |
5'-GCTCTCGGGATGTTATTCTTTGA-3' |
GR11 |
|
Q-GnRHR1 Probe |
5'-TTTACCTTCCAGTGAAATGCACCTGCG-3' |
GR12 |
|
Q-GnRHR2-F |
5'-ATCCACCAGCAGCACCTGA-3' |
GR13 |
|
Q-GnRHR2-R |
5'-ATGTCGGTGCCACTGCG-3' |
GR14 |
|
Q-GnRHR2 Probe |
5'-AGACAAAGCAGGTGAGTCGCCCCTG-3' |
GR15 |
|
Q-GnRHR3-F |
5'-TGCTACACCAGGATCTTTATTCAGA-3' |
GR16 |
|
Q-GnRHR3-R |
5'-GAGCAGCGGAGATGTGGC-3' |
GR17 |
|
Q-GnRHR3 Probe |
5'-ACAGATGACAAAAAAGAACATGCCCTCCAAT-3' |
GR18 |
All probes were labeled with 5’ FAM reporter dye and 3’ TAMARA quencher dye.