This PDF file includes:

• fig. S1. Overview of particle classification and structure determination.
  
• fig. S2. Fourier shell correlation curves and model statistics.
  
• fig. S3. Local resolution.
  
• fig. S4. Effect of the A-site hairpin on the stability of peptidyl-tRNA binding and on the action of RF1.
  
• fig. S5. The reactivity of the PTC with Pmn at 10°C.
  
• fig. S6. Conformation of the PTC.

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