Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie 1 - Fluorescent time-lapse imaging of kinetochore dynamic in a rad21-K1mutant cell. rad21-K1 mutant cells expressing Ndc80-gfp as a marker of the six kinetochores (green) and Cdc11-cfp as a marker of the two SPBs (red) were recorded by time-lapse wide field microscopy. MAARS detected the occurrence of merotelic attachment and its correction. Frames were collected every 20 sec. Frame rate= 3 frames/min. Representative frames from this video are shown in Figure 6.
- Movie 2 - : Fluorescent time-lapse imaging of kinetochore dynamic in a wild type cell. Wild-type cells expressing Ndc80-gfp as a marker of the six kinetochores (green) and Cdc11-cfp as a marker of the two SPBs (red) were recorded by time-lapse wide field microscopy. MAARS detected the occurrence of a rare event of chromosome segregation defect. Frames were collected every 20 sec. Frame rate= 3 frames/min. Representative frames from this video are shown in Supplemental Figure S5.
- Movie 3 - : Fluorescent time-lapse imaging of kinetochore dynamic in a wild type cell. Wild-type cells expressing Ndc80-gfp as a marker of the six kinetochores (green) and Cdc11-cfp as a marker of the two SPBs (red) were recorded by time-lapse wide field microscopy. MAARS detected the occurrence of a rare event of chromosome segregation defect. Frames were collected every 20 sec. Frame rate= 3 frames/min. Representative frames from this video are shown in Figure 7.