Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Video 1 - TIRF time-lapse recording of a WPB positive for YFP-Munc13-4 (left panel; green in the merge panel on the right) and VWF-RFP (middle panel; red in the merge panel on the right). HUVEC were stimulated with 100 μM histamine at t=0 s. Frames were taken twice per second, display rate is five frames per second. Scale bar represents 1 μm. Stills from this recording are shown in figure 5b.
- Video 2 - TIRF time-lapse recording of a cell expressing YFP-Munc13-4 (left panel; green in the merge panel on the right) and VWF-RFP (middle panel; red in the merge panel on the right). HUVEC were stimulated with 100 μM histamine at t=0 s. Frames were taken twice per second, display rate is 21 frames per second. Scale bar represents 10 μm. Stills from this recording are shown in figure 6a.
- Video 3 - TIRF time-lapse recording of a cell expressing YFP-S100A10. HUVEC were stimulated with 100 μM histamine at t=0 s. Frames were taken twice per second, display rate is seven frames per second. Scale bar represents 10 μm. Stills from this recording are shown in figure S6a
- Video 4 - TIRF time-lapse recording of a cell expressing AnxA2-GFP. HUVEC were stimulated with 100 μM histamine at t=0 s. Frames were taken twice per second, display rate is seven frames per second. Scale bar represents 10 μm. Stills from this recording are shown in figure S6b.
- Video 5 - Confocal time-lapse recording of a cell expressing S100A10-YFP and Munc13-4-mKate. HUVEC were stimulated with 100 μM histamine at t=0 s. Frames were taken every 2.8 seconds, display rate is seven frames per second. Scale bar represents 5 μm. Stills from this recording are shown in figure S6c.