AD, Alzheimer’s disease; LB, Lewy body; LBD, LB dementia, MID, mutiple infarct

dementia; PD, Parkinson’s disease; PMD, postmortem delay. Brain tissues were obtained from the Brain Resource Center (Department of Pathology, Johns Hopkins University School of Medicine). At autopsy, one hemibrain was cut and stored at –80° C, and another hemi-brain was fixed in formalin, and tissue blocks were embedded in paraffin for histological analysis. The pathological diagnosis of AD was formulated according to the Consortium to Establish a Registry for Alzheimer's Disease (1), and PD was formulated according to the criteria proposed by McKeith et al. (2). LB/LN loads were determined by semi-quantitative analysis of a -Syn-immunostained paraffin sections. Briefly, 10-m m paraffin sections were pretreated with formic acid and immunostained with Syn-1 antibody. The LB load was determined by counting the number of LBs per ´ 100 microscopic field, corresponding to 3.14 mm², by using a light microscope interfaced with a Microbrightfield Sterology System (3). The samples in bold were used for the semiquantitative analysis.

1. Mirra, S. S., Heyman, A., McKeel, D., Sumi, S. M., Crain, B. J., Brownlee, L. M., Vogel, F. S., Hughes, J. P., van Belle, G. & Berg, L. (1991) Neurology 41, 479–486.

2. McKeith, I. G., Galasko, D., Kosaka, K., Perry, E. K., Dickson, D. W., Hansen, L. A., Salmon, D. P., Lowe, J., Mirra, S. S., Byrne, E. J. et al. (1996) Neurology 47, 1113–1124.

3. Pletnikova, O., West, N., Lee, M. K., Rudow, G., Dawson, T. M., Marsh, L. & Troncoso, J. C. (December 28, 2004) Neurobiol. Aging, 10.1016/j.neurobiolaging.2004.10.006.