Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Video 1 - FRET-based Rac1 biosensor exposed to long-term laminar flow shows downstream Rac1 activity. HUVECs were transfected with the FRET-based biosensor, exposed to long-term flow (12h, 10 dynes/cm2). Movie shows spatio-temporal activation of Rac1 (Venus/Cer3) in time at the downstream side of the cell. Black/dark colors represent low Rac1 activity, whereas white/red colors represent high Rac1 activity. Arrow indicates the flow direction.
- Video 2 - Trio-deficient cells show reduced polarization of downstream Rac1 activity upon exposure to long-term flow. HUVECs were first transduced with shRNA targeting Trio, containing an RFP-tag and are subsequently transfected with the FRET-based Rac1 biosensor. Next, cells were exposed to long-term flow (12 h, 10 dynes/cm2). Movie shows spatio-temporal activation of Rac1 (Venus/Cer3), albeit reduced at the downstream side. Black/dark colors represent low Rac1 activity, whereas white/red colors represent high Rac1 activity. Arrow indicates the flow direction. shTrio-tagRFP shows visualizes knock-down cells.