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Fig. 5. p165 characterization and isolation. (A) Neurotoxicity of different amyloid-b(Ab ) forms (2 days of treatment): control, untreated cortical culture; tox, Ab(1–40) treatment by consequent application of seed and soluble as described in Materials and Methods; tox1, alternative treatment with Ab(1–40) (see Materials and Methods); tox42, Ab(1–42) treatment; seed, treatment with "seed" only; soluble, treatment with "soluble" only. (B) Whole-cell lysate pY₈₄₅EGFR Western blot of cortical cultures treated for 6 h with different preparations of Ab as described in A. (C) Schematic presentation of the purification procedure. (D) pY₈₄₅EGFR Western blot of aliquots from the purification steps.

Fig. 6. ADAM12 expression is not protective against FISH_348–1105 toxicity. Inverted images of GFP-positive cells expressing FISH_348–1105 (all images) and ADAM12 or ADAM12D MP deletion mutant as shown.

Fig. 7. Adenovirus-mediated expression of full-length, but not metalloprotease-deficient, ADAM12, causes neurotoxicity. Inverted image of GFP-positive cells expressing ADAM12 and ADAM12D MP is shown. Images were taken at the indicated postinfection times.

Fig. 8. ADAM12 cleavage is induced by FISH_348-1105 expression. ADAM12D MP blocks this cleavage. Shown is an ADAM12 (N-terminal) Western blot of conditioned medium from cortical cultures expressing FISH_348-1105 and ADAM12D MP mutants as indicated. Arrow indicates p32 fragment.