Genome Stability in Engineered Strains of the Extremely Thermophilic Lignocellulose-Degrading Bacterium Caldicellulosiruptor bescii

Supplemental material

• Supplemental file 1 -

  Plasmid pGR002 used in construction of MACB1013 (Fig. S1), cultures of strains (Fig. S2), ISCbe4 element insertion into a replicating shuttle vector (Fig. S3), Southern blots (Fig. S4 and S7), gel for PCR results (Fig. S5), overall genome arrangements (Fig. S6), chromosomal NCBI/JGI contig name, length, and public accession number for each strain (Table S1), and genes likely disrupted by IS elements in sequenced strains (Table S5).

  PDF, 2.7M

• Supplemental file 2 -

  Comparison of the eight PacBio-sequenced strains to the reference sequence (Tables S2 to S4).

  XLSX, 45K