Supporting Information

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Fig. 8. Amino acid sequence alignment of the 52 putative Myxococcus xanthus enhancer-binding activator proteins (EBPs) with the central s ⁵⁴ interaction domain (amino acids 140–369) of Escherichia coli NtrC (P07613). The sequences were aligned by using CLUSTALW (www.ebi.ac.uk/clustalw) with default settings; the alignment is presented by GENEDOC (www.psc.edu/biomed/genedoc). Residues on black, dark gray, and light gray backgrounds indicate 100%, 80%, and 60% amino acid similarity, respectively. The seven conserved regions (C1–C7) and the GAFTGA motif in the s ⁵⁴ interaction domain are indicated above the alignment.

Fig. 9. Domain architecture of the 52 putative Myxococcus xanthus enhancer-binding activator proteins (EBPs). Characterization of protein domains was done by using the Pfam hidden Markov model (HMM) database (http://pfam.wustl.edu/hmmsearch.shtml). Domains are color-coded as follows: green, FHA domains (Pfam accession no. PF00498); yellow, GAF domains (Pfam accession no. PF01590); blue, s ⁵⁴-dependent EBP domains (Pfam accession no. PF00158); red, and helix-turn-helix (HTH) DNA-binding domains (Pfam accession no. PF02954); purple, v4r (vinyl 4 reductase) domains (Pfam accession no. PF02830); dark blue, XylR_N domains (Pfam accession no. PF06505; XylR_N domains are usually found adjacent to v4r domains); black, RtcR domains (Pfam accession no. PF06956); grey, response regulator receiver domains (Pfam accession no. PF00072). For seven EBPs, no N-terminal sensory domain could readily be identified. (Scale bar, 100 aa.)

Fig. 10. Disruption of M4885 does not affect motility. Cells to be tested for motility were spotted on casitone-Tris agar plates. The colony swarm edges were recorded after 24 h. Strains that carry both an A^– mutation and an S^– mutation are nonswarming. The following strains were used: DK1217 (aglB1), DK10410 (D pilA), DK12730 (aglB1, Mx4885::pJEL4885.8), and DK12731 (D pilA, Mx4885::pJEL4885.8).

Fig. 11. Disruption of M4885 does not affect A-signal production. Measurement of extracellular complementation of b -galactosidase expression in an A-signaling defective tester strain (DK4324, asgB W4521) by codevelopment with A-signal donor strains. Tester and donor strains were mixed 1:1 and induced to develop. Samples were collected at the indicated time points and assayed for specific activity b -galactosidase, which is given as nanomoles of o-nitrophenol produced per minute per milligram of total protein. The A-signal donor strains used were wild type DK1622 (asgB⁺) (filled squares), DK12702 (Mx4885::Km) (open squares), and DK4324 (asgB) (filled circles).