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Fig. 5. RepA binding to different iteron-pairs. (A) EMSA of binding of WT and mutant RepAs to fragments carrying iteron pairs nos. 14 and 13 in their natural disposition as shown in the top and in Fig. 1A. (B) Same as in A, except that the iteron pairs are nos. 11 and 10. (C) Graphical representation of percentage of fragments carrying iterons nos. 14 and 13 with 1- and 2-bound RepAs. (D) Same as in C except that the iterons are nos. 11 and 10.

Fig. 6. Steady-state monomer level at different plasmid concentrations. Fraction of RepA as dimer or fraction of promoter repressed is denoted by r. The r is 0 for linear and 1 for the square- and fourth-root plots. The square-root plot applies when transcriptional autorepression or inactivation by dimerization is present alone. Combining the two generates the fourth-root plot.