Cooperative Metabolism in a Three-Partner Insect-Bacterial Symbiosis Revealed by Metabolic Modeling

Supplemental material

• Supplemental file 1 -

  Fig. S1 (Overview of Portiera and Hamiltonella genomes), S2 and S4 (Metabolic inputs to [S2] and outputs from [S4] bacterial compartments of 3-species integrated metabolic model), S3 (Concentrations of shared metabolites and biomass production), S5 (Precursor availability and essential amino acid production), and S6 (Cooperative pantothenate synthesis involving shared pathways between Bemisia and Portiera) and legends to Tables S1 to S8

  PDF, 6.2M

• Supplemental file 2 -

  Table S1 (Stand-alone Portiera metabolic model)

  XLS, 154K

• Supplemental file 3 -

  Table S2 (Stand-alone Hamiltonella metabolic model)

  XLS, 333K

• Supplemental file 4 -

  Table S3 (Stand-alone Bemisia metabolic model)

  XLS, 210K

• Supplemental file 5 -

  Table S4 (Three-compartment Portiera-Hamiltonella-Bemisia model)

  XLS, 525K

• Supplemental file 6 -

  Table S5 (Relative densities of Portiera and Hamiltonella)

  XLSX, 12K

• Supplemental file 7 -

  Table S6 (Objective function components in metabolic models and amino acid proportions)

  XLS, 8.9M

• Supplemental file 8 -

  Table S7 (Single gene deletions for stand-alone metabolic models)

  XLS, 376K

• Supplemental file 9 -

  Table S8 (Three-compartment metabolic model predicted reaction fluxes and inputs, outputs, and predicted fluxes)

  XLSX, 35K