This PDF file includes:

• Legends for movies S1 and S2
• table S1. Volume statistics of all the broken chromosomes in the nucleus.
• table S2. Reassignment of all the intact chromosomes as a comparison with the assignment in the main text.
• fig. S1. SBFSEM slices of the measured (smaller) chromosomes.
• fig. S2. Second linear fitting of the chromatid volumes against their base pair numbers.

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Other Supplementary Material for this manuscript includes the following:

• movie S1 (.mpg format). 3D rendering of the measured prophase nucleus.
• movie S2 (.mpg format). 3D rendering of the measured prophase nucleus from another orientation.