Table S1 (Comparison of methods for protein concentration determination) and Fig. S1 (Sequence alignment of BosR with Fur family members), S2 (Evolutionary relationships of Fur family members), S3 (Predicted model of BosR), S4 (Expression and purification of recombinant BosR), S5 (Molecular weight determination of BosR), S6 [Cu(II) and Zn(II) have a higher affinity than Fe(II) for the regulatory sites of BosR], S7 [EDTA could reverse the inhibitory effect of Zn(II) on BosR activity but not that of Cu(II)], S8 [Cu(II) has a higher affinity than Zn(II) for the regulatory sites of BosR], S9 (Sequence specificity of BosR binding to FPospAB), and S10 (Effects of DTT, TCEP, and EDTA on BosR DNA-binding activity)
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