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Fig. 7. PDGF-CC stimulates DNA replication of HSC but not in human or murine hepatocyte cultures. (A) (³H)thymidine incorporation into rat liver stellate cells (CFSC-2G) was measured after exposure to the indicated concentrations of PDGF-CC or PDGF-BB. (B) (³H)thymidine incorporation into murine hepatocyte cultures, AML-12 (red bars), or human hepatocyte cultures, HH4 (blue bars) was measured after exposure to the indicated growth factor (final concentration 10 ng/ml or 10% FCS). The mean ± SEM for triplicate wells is shown.

Fig. 8. Hepatic fibrosis in mice treated with PDGF-C (B and D) or GFP (A and C). (A and B) Hematoxylin/eosin-stained liver sections of mice treated with PDGF-C or GFP. (C and D) Immunohistochemistry (IHC) staining for a-SMA. Original magnification is ´ 400.

Fig. 9. Quantification of PDGF-CC levels in Tg by ELISA. Serum from transgenic (Tg) (filled circles) and WT (open circles) mice was collected at the indicated times, and levels of active PDGF-CC were measured by ELISA. Data are presented as the mean ± SEM for four or more mice.