Table 1. Primary and secondary antibodies used
|
Antigen |
Primary antibody |
Secondary antibody |
Procedure |
|
EGFP |
Rabbit polyclonal (Molecular Probes) |
FITC-conjugated donkey anti-rabbit or FITC-conjugated goat anti-rabbit |
IHC |
|
a -sarcomeric actin |
Mouse monoclonal (Sigma) |
Cy5- or TRITC-conjugated donkey anti-mouse |
IHC |
|
Troponin T |
Goat polyclonal (Santa Cruz Biotechnology) |
Texas-red-conjugated bovine anti-goat or Cy5-conjugated donkey anti-goat |
IHC |
|
Troponin I |
Goat polyclonal (Santa Cruz Biotechnology) |
Cy5- or TRITC-conjugated donkey anti-goat |
IHC |
|
a -smooth muscle actin |
Mouse monoclonal (Sigma) |
TRITC-conjugated donkey anti-mouse |
IHC |
|
GATA-4 |
Rabbit polyclonal (Santa Cruz Biotechnology) |
FITC-conjugated donkey anti-rabbit |
IHC |
|
MEF2C |
Rabbit polyclonal (Santa Cruz Biotechnology) |
TRITC-conjugated donkey anti-rabbit |
IHC |
|
Cardiac myosin heavy chain |
Mouse monoclonal (Chemicon) |
Cy5- or TRITC-conjugated donkey anti-mouse |
IHC |
|
Connexin 43 |
Rabbit polyclonal (Sigma) |
TRITC-conjugated donkey anti-rabbit |
IHC |
|
N-cadherin |
Rabbit polyclonal (Santa Cruz Biotechnology) |
FITC-conjugated donkey anti-rabbit |
IHC |
|
Chromosome 12 |
Rat chromosome/Y Paint Probe (Cambio) |
None |
FISH |
|
Collagen type I |
Goat polyclonal (Santa Cruz Biotechnology) |
FITC-conjugated bovine anti-goat |
IHC |
|
Collagen type III |
Goat polyclonal (Santa Cruz Biotechnology) |
FITC-conjugated bovine anti-goat |
IHC |
|
von Willebrand factor |
Goat polyclonal (Santa Cruz Biotechnology) |
TRITC-conjugated donkey anti-goat |
IHC |
|
GATA-6 |
Goat polyclonal (Santa Cruz Biotechnology) |
TRITC-conjugated donkey anti-goat |
IHC |
|
TER-119 |
Rat polyclonal (PharMingen) |
Cy5-conjugated donkey anti-rat |
IHC |
|
BrdUrd |
Mouse monoclonal (Roche Molecular Biochemicals) |
FITC-conjugated donkey anti-mouse |
IHC |
|
Ki67 |
Rabbit polyclonal (NovoCastra, Newcastle, U.K.) |
FITC-conjugated donkey anti-rabbit |
IHC |
|
MCM5 |
Mouse monoclonal (Accurate Chemicals) |
FITC or TRITC-conjugated donkey anti-mouse |
IHC |
|
c-kit |
Mouse monoclonal (DAKO) |
FITC- or TRITC-conjugated donkey anti-mouse |
Flow cytometry |
|
Rhodamine-labeled dextran |
Sigma |
None |
Two-photon microscopy |
|
DAPI |
Sigma |
None |
IHC |
|
Propidium iodide |
Sigma |
None |
IHC |
|
CXCR4 |
Rabbit polyclonal (Santa Cruz Biotechnology) |
Anti-rabbit |
WB |
IHC, immunohistochemistry; WB, Western immunoblotting.