Structural and functional analyses of pyroglutamate-amyloid-β-specific antibodies as a basis for Alzheimer immunotherapy

Supplemental Data

• Supplementary Information (.pdf, 2.6 MB) - Figure S1: Western blot analyses of AβpE3-specific antibodies Figure S2 : Measurement of binding affinities to AβpE3-18 by ITC Figure S3 : Determination of KD values for Aβ peptides, octamers and fibrils using SPR Table S1: Antibody/Fab binding analysis using ITC Figure S4: Omit electron density maps for the bound AβpE3 peptides together with overall views of the AβpE3-binding pockets Figure S5 : Comparison of AβpE3-binding mode to Fab fragment c#6 with published Fab Aβ complex structures Figure S6 : Geometry of the ?pEF-head? Figure S7 : Antibody digestion and Fab-fragment purification Supplementary experimental details: Surface Plasmon Resonance (SPR) Table S2: Amino acid residues involved in noncovalent interactions with the AβpE3-peptide (calculated from the structure with software MOE 2010)