A Natural Vibrio parahaemolyticus ΔpirA^Vp pirB^Vp+ Mutant Kills Shrimp but Produces neither Pir^Vp Toxins nor Acute Hepatopancreatic Necrosis Disease Lesions

Supplemental material

• Supplemental file 1 -

  RT-PCR and PCR assays of DNase-treated RNAs from indicated V. parahaemolyticus isolates, using duplex primers targeting pirA^Vp and pirB^Vp genes (Fig. S1); sensitivity of the Western blot assay using serially diluted total protein from the 80% ammonium sulfate precipitate fraction from 5HP and recombinant PirB^Vp (rPirB^Vp); details of the cumulative mortality of P. vannamei challenged with V. parahaemolyticus 5HP and XN87 isolates (Table S1).

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