Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Supplemental Data File 1 - Excel table of all individual rupture force values. All individual force values for ruptures at the SPB‐microtubule interface, failures at the bead‐microtubule interface, and for events that reached the load‐limit of the trap are reported in this Excel spreadsheet file.
- Movie 1 - Polarity of a reconstituted SPB‐microtubule attachment tested using kinesin motility. A kinesin‐bead bound to an SPB‐attached microtubule in the presence of ATP moves away from the SPB, demonstrating that the microtubule is attached to the SPB via its minus end, as in the physiological situation.
- Movie 2 - Rupture of an SPB‐microtubule attachment. An SPB‐attached microtubule is initially placed under compression, because the laser trap (not visible) is pushing the microtubule‐bound bead toward the SPB. (Due to the compressive force, the microtubule is initially buckled.) Then the direction of force is reversed and the magnitude of tension is gradually increased, until the SPB‐microtubule interface ruptures.